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To investigate the influence of distillation time on the extraction yield, chemical composition and antioxidant activity of essential oil from Pogostemon cablin (Blanco) Benth., essential oil from Pogostemon cablin (Blanco) Benth. growing in Guangxi with the 0~10, 10~30, 30~60, and 60~360 min distillation were obtained through hydrodistillation, analyzed by GC-MS for chemical composition, and evaluated via 1,1-diphenyl-2-trinitrophenylhydrazine free radical (DPPH•) and diammonium 2,2'-azino-bis(3-thylbenzothiazoline-6-sulfonic acid) cationic free radical (ABTS+•) scavenging assay for the antioxidant capacity. The results showed that the essential oil collected at 0~10, 10~30, 30~60 and 60~360 min distillation periods exhibited an extraction rate of 0.14%±0.01%, 0.18%±0.01%, 0.20%±0.02%, and 0.57%±0.03%, respectively, while displayed same main components belonging to sesquiterpenoids, including patchouli alcohol, patchoulone, α-bulnesene and seychellene. Among them, patchouli alcohol was of the highest relative content, with the relative content in the four distillation periods of 49.22%, 50.82%, 50.47%, and 39.07%, respectively. The median inhibition concentration (IC50) of essential oils for DPPH•were 21.75, 12.45, 12.03, and 3.54 g/L, while those for ABTS+• were 13.91, 8.62, 8.00, and 3.02 g/L, respectively. The essential oil from Pogostemon cablin (Blanco) Benth collected at 60~360 min exhibited the strongest DPPH• and ABTS+• scavenging capacities among the four distillation periods, while that collected at 0~10 min showed the weakest.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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