PDF
Full
Outline
Objective To investigate whether Huaier can reverse the drug resistance of CEM-C1 cells to glucocorticoid(GC) in human acute T lymphocytic leukemia (T-ALL) and its possible mechanism. Methods CEM-C1 and CEM-C7 cells were set as control group (0 μg/ml) and experimental groups (200 and 600 μg/ml) after treated with different concentrations of Huaier; T-ALL CEM-C1 cells were set as Huaier (100 μg/ml) combined with dexamethasone (DEX)(12.5, 25, 50, 100, 200 μg/ml)groups; CCK-8 was used to detect the affect of Huaier on the inhibition rate to proliferation of each group of cells; Flow cytometry(FCM) was employed to detect the effect of different concentrations Huaier (200 and 600 μg/ml) on the apoptosis and cell cycle of CEM-C1 and CEM-C7 cells; Western blotting was performed to detect the MDR1 and Pim3 protein expression levels in CEM-C1, CEM-C7 cells and CEM-C1 cells treated with different concentrations of Huaier (200 and 600 μg/ml); qRT-PCR was used to detect the expression level of Pim3 mRNA in CEM-C1 and CEM-C7 cells and CEM-C1 cells treated with different concentrations of Huaier(200 and 600 μg/ml). Results Huaier inhibited the proliferation of CEM-C1 and CEM-C7 cells in a dose-dependent manner;For CEM-C1 cells, compared with DEX alone, the drug resistance was reversed by 1.34 times in Huaier+DEX group, and Huaier(100 μg/ml) combined with low concentration of DEX (12.5, 25, 50 μg/ml) increased the proliferation inhibition rate of CEM-C1 cells (P<0.05); the coeffcient of drug interaction (CDI) values of all groups were <1. Compared with control group, the apoptosis rate of CEM-C1 and CEM-C7 cells increased significantly with the increase of Huaier concentration (P<0.05). Compared with control group, the number of CEM-C1 and CEM-C7 cells decreased in G0/G1 stage and increased in S stage in 200 and 600 μg/ml Huaier groups. The expression levels of MDR1 and Pim3 protein and Pim3 mRNA in CEM-C1 cells were higher than those of CEM-C7 cells (2.41±0.32 vs. 1.34±0.43; 0.39±0.10 vs. 0.13±0.05; 1.00 vs. 0.37±0.02, respectively, P<0.05). Compared with control group, the expression levels of MDR1 and Pim3 proteins and Pim3 mRNA reduced obviously in CEM-C1 cells after treatment with 200 and 600 μg/ml Huaier (P<0.05). Conclusion Huaier can inhibit the proliferation and promote the apoptosis of CEM-C1 and CEM-C7 cells, and partially reverse the resistance of CEM-C1 cells to GC, the mechanism may be related to the down-regulation of Pim3.
PDF
194
72
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62199257
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
