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Mid-term and long-term simulated microgravity causes calcification of common carotid artery in rats
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Huan Liu1, Ning-Yu Ru1, Qiang Lv2, Chi-Hua Guo1, Yu-Fang Guo1, Shuang Wang1, Xing-Li Su1, *, Jin Ma2, *
Medical Journal of Chinese People’s Liberation Army | 2021, 46(1) : 1 - 6
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Medical Journal of Chinese People’s Liberation Army | 2021, 46(1): 1-6
• Basic Research •
Mid-term and long-term simulated microgravity causes calcification of common carotid artery in rats
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Huan Liu1, Ning-Yu Ru1, Qiang Lv2, Chi-Hua Guo1, Yu-Fang Guo1, Shuang Wang1, Xing-Li Su1, *, Jin Ma2, *
Affiliations
  • 1Department of Basic Medicine, Xi'an Medical University, Xi'an 710021, China
  • 2Department of Aerospace Physiology, Air Force Medical University, Xi'an 710032, China
Published: 2021-01-28 doi: 10.11855/j.issn.0577-7402.2021.01.01
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Objective To explore the effects of mid-term (4 weeks) and long-term (8 weeks) simulated microgravity on calcification of the common carotid artery in the rats. Methods Hindlimb-unweighted (HU) rat model was used to simulate the effects of weightlessness on the cardiovascular system. Seventy-five male SD rats were randomized into the control group, 4-week HU group (HU4w), and 8-week HU group (HU8w). After the common carotid arteries were separated, the quantification of calcium content and the alizarin red staining were used to detect the calcium salt deposition in the common carotid arteries. The alkaline phosphatase (ALP) activity of common carotid arteries was measured. Western blotting and the real-time polymerase chain reaction(RT-PCR) were performed to measure the protein and mRNA levels of Runx2, Msx2, BMP2, osteocalcin, and SM22α. Results Compared with the control group, the calcium content and the calcium salt deposition were increased, meanwhile, the ALP activity was enhanced in the common carotid arteries of 4-week and 8-week HU rats. Besides, the protein and mRNA levels of Runx2, Msx2, BMP2, osteocalcin were increased, while those of SM22α were decreased in the common carotid arteries of 4-week and 8-week HU rats (P<0.05 or P<0.01). Interestingly, there was no statistically significant difference of above tests between the HU4w and HU8w groups. Conclusion Mid- and long-term simulated microgravity induced the osteogenic transdifferentiation of smooth muscle cells and calcification in the common carotid artery of rats, while the degree of calcification did not change significantly with the prolonged period of simulated microgravity.

simulated microgravity  /  rats  /  common carotid artery  /  vascular calcification  /  vascular smooth muscle cells
Huan Liu, Ning-Yu Ru, Qiang Lv, Chi-Hua Guo, Yu-Fang Guo, Shuang Wang, Xing-Li Su, Jin Ma. Mid-term and long-term simulated microgravity causes calcification of common carotid artery in rats[J]. Medical Journal of Chinese People’s Liberation Army, 2021 , 46 (1) : 1 -6 . DOI: 10.11855/j.issn.0577-7402.2021.01.01
Funding
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  • National Natural Science Foundation of China(81601637)
  • National Natural Science Foundation of China(81800211)
  • Key Research and Development Program of Shaanxi(2020SF-156)
  • Natural Science Basic Research Program of Shaanxi(2016JQ8001)
  • Doctoral Research Start-Up Fund of Xi'an Medical University(2017DOC16)
Appendix
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Year 2021 volume 46 Issue 1
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173
65
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Article Info
doi: 10.11855/j.issn.0577-7402.2021.01.01
  • Receive Date：2020-07-15
  • Online Date：2025-12-26
  • Published：2021-01-28
Article Data
Affiliations
History
  • Received：2020-07-15
  • Revised：2020-12-22
Funding
National Natural Science Foundation of China(81601637)
National Natural Science Foundation of China(81800211)
Key Research and Development Program of Shaanxi(2020SF-156)
Natural Science Basic Research Program of Shaanxi(2016JQ8001)
Doctoral Research Start-Up Fund of Xi'an Medical University(2017DOC16)
Affiliations
    1Department of Basic Medicine, Xi'an Medical University, Xi'an 710021, China
    2Department of Aerospace Physiology, Air Force Medical University, Xi'an 710032, China

Corresponding:

*Su Xing-Li, E-mail: ;
Ma Jin,
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表12种不同金属材料的力学参数
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小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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