PDF
Full
Outline
Objective To investigate the effects of advanced pregnancy on DNA methylation level of hippocampus in the offspring rats. Methods 12- and 3-month-old female SD rats were mated with 3-month-old male SD rats. The offspring(s)from older mother were marked as AMA group, and those from younger mother were marked as control group. The brain and hippocampus samples of the offsprings were taken at the 7th, 14th and 28th day after birth. The mRNA levels of DNA methyltransferase (Dnmt)1, Dnmt3A and Dnmt3B were detected by real-time fluorescent quantitative PCR (RT-PCR). The protein expression levels of Dnmt1, Dnmt3A and Dnmt3B were measured by Western blotting. Immunofluorescence and ELISA were used to detect the localization and expression level of 5-methylcytosine (5-mC) in hippocampus. Results RT-PCR showed the Dnmt1 mRNA level was markedly lower in hippocampus of AMA group than that in control group on day 28 after birth (P<0.05); Western blotting analysis showed the expression level of Dnmt3A protein was obviously higher in hippocampus of the AMA group than that in control group on day 7 after birth (P<0.05), while the expression levels of Dnmt1 and Dnmt3A were lower in AMA group than those in control group on day 28 after birth (P<0.05). Immunofluorescence test showed that the 5-mC level in hippocampus was significantly higher in AMA group than that in control group on day 7 and 14 after birth (P<0.05). ELISA showed that the 5-mC level in hippocampus increased slightly but without statistical significance in AMA group than that in control group on day 7, 14 and 28 after birth (P>0.05). Conclusion Advanced pregnancy may lead to the expression disorder of Dnmts and the increased DNA methylation level in offspring's hippocampus, which may be one of the potential mechanisms of offspring's brain dysfunction.
PDF
126
43
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62199257
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
