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Objective To explore the analgesic effect of low-intensity exercise induced by interleukin (IL)-4 on chronic constriction injury of the sciatic nerve (CCI) mice. Methods A total of 48 male C57/BL6 mice were randomized into 6 groups(n=8): sham/sedentary group (SS group), sham/normal saline/exercise group (SNE group), sham/anti-IL-4/exercise group (SAE group), CCI/sedentary group (CS group), CCI/normal saline/exercise group (CNE group), CCI/anti-IL-4/exercise group (CAE group). After inducing CCI, mice were given low-intensity exercise from 1 to 15 days. For the SAE group and the CAE group, mice received 50 μg of IL-4 receptor blocker (IL-4Ra) 30 min before exercise through intraperitoneal injection. Behavioural tests were performed on the 3rd day (T1), 7th day (T2), 11th day (T3) and 15th day (T4) to analyze the difference of hyperalgesia. On the 15th day after CCI induction, the injured sciatic nerve and lumbar spinal cord (L1-L6) of all mice were collected. The levels of IL-4, IL-1Ra, IL-5, TNF-α, IL-1β, brain-derived neurotrophic factor (BDNF) and β-nerve growth factor (β-NGF) were detected by ELISA. Results The paw withdrawal mechanical threshold (PWMT) and paw withdrawal thermal latency (PWTL) values of the CNE group at T3 and T4 were higher than those of the CS group and CAE group (P<0.05); the IL-4 levels of the sciatic nerve and lumbar spinal cord in CNE group and CAE group were higher than those of CS group (P<0.05); the IL-1Ra and IL-5 levels of the sciatic nerve and lumbar spinal cord in CNE group were higher than those of CS group and CAE group (P<0.05); the TNF-α, IL-1β, BDNF and β-NGF levels of the sciatic nerve and lumbar spinal cord of CNE group were lower than those of CS group and CAE group (P<0.05). Conclusion Low-intensity exercise exerts the analgesic effect on mice with chronic constriction injury of sciatic nerve through IL-4.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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