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IR-820 loaded phase transition nanoparticles for dual-modal imaging and photodynamic effect on fibroblast-like synoviocytes of rheumatoid arthritis in vitro
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Zhu-Xi Liang1, Xin-Yu He1, Shu-Fang Chang2, Zhi-Gang Wang3, Lan Hao3, Fa-Qi Li3, Shen-Yin Zhu1, *
Medical Journal of Chinese People’s Liberation Army | 2021, 46(5) : 425 - 431
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Medical Journal of Chinese People’s Liberation Army | 2021, 46(5): 425-431
• Basic Research •
IR-820 loaded phase transition nanoparticles for dual-modal imaging and photodynamic effect on fibroblast-like synoviocytes of rheumatoid arthritis in vitro
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Zhu-Xi Liang1, Xin-Yu He1, Shu-Fang Chang2, Zhi-Gang Wang3, Lan Hao3, Fa-Qi Li3, Shen-Yin Zhu1, *
Affiliations
  • 1Department of Pharmacy, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China
  • 2Department of Obstetrics and Gynecology, the Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China
  • 3Chongqing Key Laboratory of Ultrasound Molecular Imaging, Chongqing 400010, China
Published: 2021-05-28 doi: 10.11855/j.issn.0577-7402.2021.05.01
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Objective To investigate the photodynamic effect of IR-820 and perfluoropentane (PFP) loaded phase-transition multifunctional molecular imaging probe (IR-820/PFP@PLGA NPs) on ultrasound/photoacoustic dual-mode imaging and fibroblast-like synoviocytes (FLSs) of rheumatoid arthritis (RA) in vitro. Methods The IR-820/PFP@PLGA nanoparticles were prepared by double emulsification method. The basic characteristics, thermal phase transition and photothermal effect of IR-820/PFP@PLGA nanoparticles were determined, the ultrasound/photoacoustic dual-mode imaging ability in vitro of the IR-820/PFP@PLGA nanoparticles were observed, and the generation of reactive oxygen species in vitro were evaluated. The photodynamic therapy effect of the nanoparticles on fibroblast-like synoviocytes was observed by CCK-8 method and flow cytometry. Results The prepared IR-820/PFP@PLGA nanoparticles were dark green, with a particle size of (261.8±3.2) nm and a potential of (–27.4±0.7) mV. The size of the IR-820/PFP@PLGA nanoparticles were uniform and the morphology were regular. The size of nanoparticles and the temperature of nanoparticle solution increased obviously by laser irradiation, it was positively correlated with the concentration of nanoparticles, and enhanced ultrasound imaging was observed. The photoacoustic imaging effect of nanoparticle was linearly correlated with the concentration of IR-820/PFP@PLGA nanoparticles; The singlet oxygen sensor green(SOSG) assay revealed that the nanoparticles produced a large amount of reactive oxygen species after laser irradiation with time-dependent manner; it was found by in vitro cell experiments that the nanoparticles had good biological safety, while the cytotoxicity increased along with the advanced nanoparticle concentration after laser irradiation, and also the apoptosis increased significantly. Conclusion IR-820/PFP@PLGA nanoparticles have a good dual-mode imaging and photodynamic effect on FLSs, which can provide a new idea for the integration of diagnosis and treatment for RA.

nanoparticles  /  dual-mode imaging  /  photodynamic therapy  /  new indocyanine green (IR-820)  /  perfluoropentane  /  fibroblast-like synoviocytes
Zhu-Xi Liang, Xin-Yu He, Shu-Fang Chang, Zhi-Gang Wang, Lan Hao, Fa-Qi Li, Shen-Yin Zhu. IR-820 loaded phase transition nanoparticles for dual-modal imaging and photodynamic effect on fibroblast-like synoviocytes of rheumatoid arthritis in vitro[J]. Medical Journal of Chinese People’s Liberation Army, 2021 , 46 (5) : 425 -431 . DOI: 10.11855/j.issn.0577-7402.2021.05.01
Funding
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  • National Natural Science Foundation of China(81972439)
  • Chongqing Basic Research and Frontier Exploration Project(cstc2018jcyjAX0223)
Appendix
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Year 2021 volume 46 Issue 5
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Article Info
doi: 10.11855/j.issn.0577-7402.2021.05.01
  • Receive Date：2021-01-28
  • Online Date：2025-12-24
  • Published：2021-05-28
Article Data
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History
  • Received：2021-01-28
  • Revised：2021-03-26
Funding
National Natural Science Foundation of China(81972439)
Chongqing Basic Research and Frontier Exploration Project(cstc2018jcyjAX0223)
Affiliations
    1Department of Pharmacy, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China
    2Department of Obstetrics and Gynecology, the Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China
    3Chongqing Key Laboratory of Ultrasound Molecular Imaging, Chongqing 400010, China

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Citations
表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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