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Advances in the research of recombinase polymerase amplification technology
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Ya-Nan Wang1, 2, Chang-Guo Chen2, *
Medical Journal of Chinese People’s Liberation Army | 2021, 46(5) : 504 - 511
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Medical Journal of Chinese People’s Liberation Army | 2021, 46(5): 504-511
• Review •
Advances in the research of recombinase polymerase amplification technology
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Ya-Nan Wang1, 2, Chang-Guo Chen2, *
Affiliations
  • 1Academy of Medical Laboratory, Hebei North University, Zhangjiakou, Hebei 075000, China
  • 2Department of Clinical Laboratory Medicine, the Sixth Medical Center of Chinese PLA General Hospital, Beijing 100048, China
Published: 2021-05-28 doi: 10.11855/j.issn.0577-7402.2021.05.13
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The outbreak of coronavirus disease 2019 has made nucleic acid detection widely known. Fluorescent quantitative polymerase chain reaction (PCR) technique is the most commonly used nucleic acid detection method in this epidemic control. However, its requirements on operators, instruments and sites limited its application in some scenarios with lower resources or outside laboratories. Isothermal amplification technology, especially recombinase polymerase amplification(RPA) technology, has the advantages of mild reaction conditions, high sensitivity, excellent specificity and short reaction time, which let it has a good application prospect in the rapid detection of a variety of pathogenic microorganisms. In this paper, the development and application of RPA technology are reviewed and summarized to provide a reference for the further research and promotion of this technology.

isothermal amplification techniques  /  recombinase polymerase amplification  /  site inspection  /  epidemic prevention and control
Ya-Nan Wang, Chang-Guo Chen. Advances in the research of recombinase polymerase amplification technology[J]. Medical Journal of Chinese People’s Liberation Army, 2021 , 46 (5) : 504 -511 . DOI: 10.11855/j.issn.0577-7402.2021.05.13
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  • Key Research Topic of Military Logistics(BHJ14J005)
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Year 2021 volume 46 Issue 5
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Article Info
doi: 10.11855/j.issn.0577-7402.2021.05.13
  • Receive Date：2021-03-15
  • Online Date：2025-12-24
  • Published：2021-05-28
Article Data
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History
  • Received：2021-03-15
  • Revised：2021-04-14
Funding
Key Research Topic of Military Logistics(BHJ14J005)
Affiliations
    1Academy of Medical Laboratory, Hebei North University, Zhangjiakou, Hebei 075000, China
    2Department of Clinical Laboratory Medicine, the Sixth Medical Center of Chinese PLA General Hospital, Beijing 100048, China

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