Home Journals Articles Updates About
CN
image
收藏切换
OPN/IGF1 promotes the corticospinal tract regeneration by increasing neuron IGFR expression
收藏切换
PDF
Qian Zhang1, Kun Zhang2, Zeng-Qiang Zhang1, Jian-Yong Li1, Fang Cui1, *
Medical Journal of Chinese People’s Liberation Army | 2021, 46(10) : 955 - 960
Less
收藏切换
Medical Journal of Chinese People’s Liberation Army | 2021, 46(10): 955-960
• Basic Research •
OPN/IGF1 promotes the corticospinal tract regeneration by increasing neuron IGFR expression
Full
Qian Zhang1, Kun Zhang2, Zeng-Qiang Zhang1, Jian-Yong Li1, Fang Cui1, *
Affiliations
  • 1Department of Neurology, Hainan Hospital of Chinese PLA General Hospital, Sanya, Hainan 572013, China
  • 2Department of Neurobiology, Air Force Military Medical University, Xi'an 710032, China
Published: 2021-10-28 doi: 10.11855/j.issn.0577-7402.2021.10.01
Outline
收藏切换

Objectives To study the role and associated potential molecular mechanism of osteopontin/insulin-like growth factor 1 (OPN/IGF1) in the corticospinal tract (CST) regeneration. Methods A total of 28 adult C57BL/6 mice (6-8 weeks old) were randomized into the experimental group (injection of AAV-OPN/IGF1, n=14) and control group (injection of AAV-PLAP, n=14) after training of single pellet retrieval. After received the unilateral pyramidotomy surgery, animals received AAV-OPN/IGF1 or AAV-PLAP at the injury site. Further, we employed the movement behaviour test to evaluate spinal motor recovery at 2-week intervals till 12-week after surgery. The protein kinase Cγ (PKCγ) staining in the dorsal funiculus of the cervical spinal cord indicated surgical efficiency. Lastly, we examined the axon sprouting and expression levels of ribosomal protein S6 kinase (pS6)and insulin-like growth factor receptor (IGFR) in neurons using immunohistochemical staining to study the potential mechanism of OPN/IGF1 to promote axon prouting. Results After evaluating PKCγ staining to indicate surgery effectiveness, a total of ten mice were in the experimental group and eight in the control group. In the mouse unilateral pyramidotomy model, OPN/IGF1 has not much effect on the recovery of motor function compared with the control group. After the overexpression of OPN/IGF1 on cortical neurons, sprouting of the spared CST was increased by the elevated expression of pS6 and IGFR, and the difference was statistically significant (P<0.05). Conclusions OPN/IGF1 promotes the sprouting of the CST by increasing the expression of neuronal IGFR and pS6.

osteopontin/insulin-like growth factor 1  /  pyramidotomy  /  axon regeneration  /  insulin-like growth factor receptor
Qian Zhang, Kun Zhang, Zeng-Qiang Zhang, Jian-Yong Li, Fang Cui. OPN/IGF1 promotes the corticospinal tract regeneration by increasing neuron IGFR expression[J]. Medical Journal of Chinese People’s Liberation Army, 2021 , 46 (10) : 955 -960 . DOI: 10.11855/j.issn.0577-7402.2021.10.01
Funding
Less
  • National Natural Science Foundation of China(81901252)
  • National Natural Science Foundation of China(81601056)
  • Natural Science Foundation of Hainan Province(820QN383)
  • Natural Science Foundation of Hainan Province(2018CXTD348)
Appendix
Less
Year 2021 volume 46 Issue 10
PDF
109
39
Cite this Article
BibTeX
Article Info
doi: 10.11855/j.issn.0577-7402.2021.10.01
  • Receive Date：2020-10-25
  • Online Date：2025-12-19
  • Published：2021-10-28
Article Data
Affiliations
History
  • Received：2020-10-25
  • Revised：2021-06-20
Funding
National Natural Science Foundation of China(81901252)
National Natural Science Foundation of China(81601056)
Natural Science Foundation of Hainan Province(820QN383)
Natural Science Foundation of Hainan Province(2018CXTD348)
Affiliations
    1Department of Neurology, Hainan Hospital of Chinese PLA General Hospital, Sanya, Hainan 572013, China
    2Department of Neurobiology, Air Force Military Medical University, Xi'an 710032, China

Corresponding:

References
Share
https://castjournals.cast.org.cn/joweb/jfjyxzz/EN/10.11855/j.issn.0577-7402.2021.10.01
Share to
QR

Scan QR to access full text

Cite this article
BibTeX
Citations
表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
关闭全屏
  • BibTeX
  • EndNote
  • RefWorks
  • TxT
Links
Articles
Latest Articles
Most Read
Collections
Updates
Events
News
Multimedia
About
About Us
Contact
No. 86 Xueyuan South Road, Haidian District, Beijing
100081
010-62199257
qkjq@cast.org.cn

Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House