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Objective To investigate the effect and mechanism of total parenteral nutrition (TPN) on the myocardial injury of young rats. Methods Twenty-four male young Sprague-Dawley rats aged 6 to 8 weeks were randomly divided into 4 groups (6 each): normal group, control group, TPN-7 d group and TPN-14 d group. Rats in normal group were not placed into tubes and had free food and water. Rats in control group were catheterized through the right jugular vein and continuously infused with normal saline, free food and water. Those in TPN group were catheterized through the right jugular vein and continuously infused with TPN nutrient solution and abstained from food and water. Rats in the 4 groups were killed and their myocardial tissue were obtained on the 14th, 14th, 7th and 14th day after the establishment of the model, respectively. HE staining was performed to observe the pathological changes of myocardial tissue, TUNEL staining was used to observe the apoptosis of myocardial tissue, the contents of malondialdehyde (MDA) and hydrogen peroxide (H2O2) in myocardial tissue were detected by ELISA, the expression of myeloperoxidase (MPO) in myocardial tissue was detected by qRT-PCR, Western blotting and immunofluorescence staining. Results The 50% of myocardial tissue of rats in TPN-7 d group were slightly damaged, and all the rats in TPN-14 d group were slightly to severely damaged. Compared with normal group and control group, the cardiomyocytes apoptosis rate in the TPN groups increased significantly (P<0.05), MDA and H2O2 contents increased markedly (P<0.05), the expressions of MPO mRNA, protein and immunofluorescence increased (P<0.05). The apoptotic rate, H2O2 content, MPO protein and immunofluorescence expression were higher in TPN-14 d group than those in TPN-7 d group (P<0.05). Conclusions TPN over 1 week can cause myocardial injury in young rats, and the degree of injury increases with the extension of TPN. Oxidative stress may be one of the mechanisms of the injury.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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