Objective To investigate the effect of SE-hBCG on the immunogenicity of recombinant cytomegalovirus glycoprotein B (CgB) and freeze-dried rabies human vaccine (Rab). Methods A total of 145 SPF female BALB/c mice aged 6-8 weeks were selected for the following experiments. In experiment one, five groups of mice (five mice per group) received intramuscular injections of 0.2 ml of SE, SE-hBCG, CgB, SE+CgB or SE-hBCG+CgB three times (at week 0, 2 and 4), respectively.Two weeks after the last immunization was the endpoint of this experiment. In experiment two, four groups of mice (30 mice per group) received two intraperitoneally injections at week 0 and 1 with 0.2 ml of PBS, Rab, SE+Rab or SE-hBCG+Rab, respectively.Samples were collected at 4, 8, 10, 12, 14, 35 days after the first immunization. Spleen lymphocytes of mice were isolated after homogenizing spleens. The enzyme-linked immunosorbent spot assay (ELISPOT) was used to detect the number of antigen-specific IFN-γ and IL-4-secreting spot forming cells (IFN-γ-SFC or IL-4-SFC). Sera were harvested from eyeball blood. Antigen-specific IgG was detected using ELISA. Results SE-hBCG induced mice to produce CgB-specific Th1 responses as mice immunized with SE-hBCG produced greater numbers of IFN-γ-SFC than mice immunized with SE (266.0±87.9 vs. 104.5±28.8, P<0.05). Mice immunized with SE-hBCG adjuvanted CgB vaccines produced higher levels of CgB-specific IgG antibodies (18 800.0±2396.0) and greater numbers of IFN-γ-SFC (440.5±38.4) than mice immunized with CgB (3333.0±737.9 for CgB-specific IgG antibody titer and 189.2±21.4 for IFN-γ-SFC, P<0.05). Four days after the first immunization, SE or SE-hBCG adjuvanted Rab vaccines induced low-level anti-Rab IgG antibodies (OD₄₅₀≤0.2). Anti-Rab IgG antibodies of mice immunized with Rab, SE or SE-hBCG adjuvanted Rab peaked 12 days after the first immunization. Mice immunized with SE-hBCG adjuvanted Rab vaccines produced higher levels of anti-Rab IgG antibodies 8, 10, 14, and 35 days (1700.0±200.0, 19 400.0±1661.3, 23 000±358.9, 23 600.0±6038.2, respectively)after the first immunization than mice immunized with Rab (310.0±97.9, 6730.0±1655.4, 6000.0±1655.6, 4400.0±1655.5,respectively, P<0.05) and greater numbers of IFN-γ-SFC 4, 8, 14 and 35 days (110.8±52.5, 213.0±29.7, 105.2±33.7,80.4±36.8, respectively) after the first immunization than mice immunized with PBS (6.4±3.5, 32.2±12.9, 11.4±5.1, 4.4±2.5,respectively, P<0.05). Conclusion SE-hBCG could enhance the immunogenicity of CgB and Rab, and it is likely to become an immunopotentiator.