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Objective To investigate the expression and clinical significance of blood basophil CD16 and CD32 in asthma-chronic obstructive pulmonary disease overlap (ACO) and chronic obstructive pulmonary disease (COPD). Methods COPD and ACO patients in the Third Affiliated Hospital of Jinzhou Medical University from December 2019 to December 2020 (set as COPD group and ACO group) and recruited healthy volunteers (set as healthy control group) were selected as research objects. General information including gender, age, medical history, age at onset, body mass index (BMI) and smoking history, and peripheral venous blood were collected. The expressions of CD16 and CD32 in basophil granulocyte enriched groups were detected by flow cytometry. The expression level of tumor necrosis factor-α (TNF-α) in plasma was determined by ELISA. Results Compared with healthy control group, the proportion of CD16+CCR3+ cells, CD16+CD123+HLA-DR- cells and CD16+CCR3+CD123+HLA-DR-cells in peripheral blood mononuclear cell population of ACO group and COPD group were increased (P<0.05), the proportion of CD32+CD123+HLA-DR- cells and CD32+CCR3+CD123+HLA-DR- cells in COPD group increased (P=0.003, P=0.030), the proportion of CD32+CD123+HLA-DR- cells and CD32+CCR3+CD123+HLA-DR- cells in ACO group decreased (P=0.019, P=0.031).The CD32 mean fluorescence intensity (MFI) of CCR3+ cells and CCR3+CD123+HLA-DR- cells was lower in ACO group than in healthy control group (P=0.006, P=0.047). The plasma TNF-α expression level was higher in ACO group than in healthy control group with statistically significant difference (P=0.036). Conclusion Basophil granulocyte source CD16 and CD32 may play an important role as the pathogenesis of ACO and COPD, and TNF-α is involved in the pathogenesis of ACO, which may provide a new target for the subsequent clinical treatment of ACO and COPD.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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