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Effect of fatty acid binding protein 4 in cardiomyocyte pyroptosis induced by homocysteine
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Xiao-Yan Dong1, 2, 3, Da-Yue Liu1, 2, 3, Ling-Bo Xu1, 2, 3, Ling-Yu Gu2, 3, 4, Sheng-Chao Ma1, 2, 3, An-Ning Yang1, 2, 3, Yong Yang2, 4, 5, Yun Liu2, 4, 6, Yi-Deng Jiang1, 2, 3, *
Medical Journal of Chinese People’s Liberation Army | 2022, 47(1) : 33 - 38
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Medical Journal of Chinese People’s Liberation Army | 2022, 47(1): 33-38
• Basic Research •
Effect of fatty acid binding protein 4 in cardiomyocyte pyroptosis induced by homocysteine
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Xiao-Yan Dong1, 2, 3, Da-Yue Liu1, 2, 3, Ling-Bo Xu1, 2, 3, Ling-Yu Gu2, 3, 4, Sheng-Chao Ma1, 2, 3, An-Ning Yang1, 2, 3, Yong Yang2, 4, 5, Yun Liu2, 4, 6, Yi-Deng Jiang1, 2, 3, *
Affiliations
  • 1School of Basic Medical Sciences, Ningxia Medical University, Yinchuan 750004, China
  • 2NHC Key Laboratory of Metabolic Cardiovascular Diseases Research, Yinchuan 750004, China
  • 3Ningxia Key Lab of Vascular Injury and Repair Research, Yinchuan 750004, China
  • 4Clinical Medical College, Ningxia Medical University, Yinchuan 750004, China
  • 5Department of Nuclear Medicine, People's Hospital of Ningxia Hui Autonomous Region, Yinchuan 750004, China
  • 6Department of Radiology, General Hospital of Ningxia Medical University, Yinchuan 750004, China
Published: 2022-01-28 doi: 10.11855/j.issn.0577-7402.2022.01.0033
Outline
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Objective To investigate the effect of fatty acid binding protein 4 (FABP4) in cardiomyocyte pyroptosis induced by homocysteine (Hcy). Methods The cultured rat cardiomyocytes were divided into two groups: control group(0 mmol/L Hcy) and Hcy group (3 mmol/L Hcy). The expression of FABP4 and pyroptosis related proteins [NOD-like receptor protein 3 (NLRP3), caspase-1, interleukin-1β (IL-1β)] were detected by Western blotting. The expression level of FABP4 mRNA was detected by qRT-PCR. The changes of expression of pyroptosis associated proteins were detected by immunofluorescence staining. The cultured cardiomyocytes were treated with FABP4 inhibitor (BMS-309403), and then divided into control group (0 mmol/L Hcy), Hcy group (3 mmol/L Hcy) and Hcy+BMS-309403 group (3 mmol/L Hcy and 50 μmol/L BMS-309403), and the expression levels of pyroptosis related proteins were then re-detected. Results Compared with control group, Western blotting showed that the expression levels of FABP4, NLRP3, caspase-1 and IL-1β in Hcy group increased significantly (P<0.05); Compared with control group, qRT-PCR results showed that the expression of FABP4 in Hcy group increased significantly (P<0.05); Compared with control group, immunofluorescence staining results showed that the fluorescence intensity of NLRP3, caspase-1 and IL-1β in Hcy group increased significantly. After adding FABP4 inhibitor (BMS-309403) and Hcy treatment, the expression levels of FABP4, NLRP3, caspase-1 and IL-1β in Hcy+BMS-309403 group decreased significantly compared with Hcy group (P<0.05 or P<0.01). Conclusion FABP4 may promote cardiomyocytes pyroptosis induced by homocysteine.

cardiomyocytes  /  pyrotosis  /  homocysteine  /  fatty acid binding protein 4
Xiao-Yan Dong, Da-Yue Liu, Ling-Bo Xu, Ling-Yu Gu, Sheng-Chao Ma, An-Ning Yang, Yong Yang, Yun Liu, Yi-Deng Jiang. Effect of fatty acid binding protein 4 in cardiomyocyte pyroptosis induced by homocysteine[J]. Medical Journal of Chinese People’s Liberation Army, 2022 , 47 (1) : 33 -38 . DOI: 10.11855/j.issn.0577-7402.2022.01.0033
Funding
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  • National Natural Science Foundation of China(81860044)
  • Youth Project of National Natural Science Foundation of China(81900273)
  • Ningxia Key Research and Development Projects(2020BFH02003)
  • Ningxia Natural Science Foundation(NZ14151)
Appendix
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Year 2022 volume 47 Issue 1
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Article Info
doi: 10.11855/j.issn.0577-7402.2022.01.0033
  • Receive Date：2021-07-26
  • Online Date：2025-12-18
  • Published：2022-01-28
Article Data
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History
  • Received：2021-07-26
  • Accepted：2021-09-06
Funding
National Natural Science Foundation of China(81860044)
Youth Project of National Natural Science Foundation of China(81900273)
Ningxia Key Research and Development Projects(2020BFH02003)
Ningxia Natural Science Foundation(NZ14151)
Affiliations
    1School of Basic Medical Sciences, Ningxia Medical University, Yinchuan 750004, China
    2NHC Key Laboratory of Metabolic Cardiovascular Diseases Research, Yinchuan 750004, China
    3Ningxia Key Lab of Vascular Injury and Repair Research, Yinchuan 750004, China
    4Clinical Medical College, Ningxia Medical University, Yinchuan 750004, China
    5Department of Nuclear Medicine, People's Hospital of Ningxia Hui Autonomous Region, Yinchuan 750004, China
    6Department of Radiology, General Hospital of Ningxia Medical University, Yinchuan 750004, China

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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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