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Role of CYFIP2 in proliferation and migration of renal clear cell carcinoma cells
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Zhe-Kun An1, 2, Xiu-Bin Li2, Chen-Yu Lin2, Xin-Xin Guo2, Xiao-Long Cheng1, *, Ming Cai3, 4, *
Medical Journal of Chinese People’s Liberation Army | 2022, 47(1) : 26 - 32
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Medical Journal of Chinese People’s Liberation Army | 2022, 47(1): 26-32
• Basic Research •
Role of CYFIP2 in proliferation and migration of renal clear cell carcinoma cells
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Zhe-Kun An1, 2, Xiu-Bin Li2, Chen-Yu Lin2, Xin-Xin Guo2, Xiao-Long Cheng1, *, Ming Cai3, 4, *
Affiliations
  • 1School of Basic Medical, Shanxi Medical University, Taiyuan 030001, China
  • 2Institute of Respiratory Sciences, the Eighth Medical Center of Chinese PLA General Hospital, Beijing 100091, China
  • 3Department of Urology, the Second Affiliated Hospital of School of Medicine, Zhejiang University, Hangzhou 310009, China
  • 4Department of Urology, the Third Medical Center of Chinese PLA General Hospital, Beijing 100143, China
Published: 2022-01-28 doi: 10.11855/j.issn.0577-7402.2022.01.0026
Outline
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Objective To investigate the role of CYFIP2 gene in proliferation and migration of renal clear cell carcinoma(KIRC). Methods The TCGA database was used to analyze the expression of CYFIP2 gene in KIRC and the correlation between the expression and clinicopathological factors. The KIRC cell line 786-O was transfected with siRNA-CYFIP2 to knockdown the CYFIP2 gene, and the CYFIP2 gene stable overexpression system was constructed by infecting 786-O cells with viral particles carrying the CYFIP2 gene. The 786-O cells were used as the knockdown group (siRNA-CYFIP2, si-CYFIP2) after the CYFIP2 gene was knockdown, and set the corresponding control group si-NC; the 786-O cells were used as the overexpression group (Over Expression-CYFIP2, OE-CYFIP2) after performing CYFIP2 gene overexpression, and set the corresponding control group OE-NC.Subsequently, cell proliferation assays and Transwell assays were performed to analyze the differences in proliferative activity and migration ability of 786-O cells after knockdown or overexpression of CYFIP2 gene. Results The expression of CYFIP2 in KIRC was significantly lower (P<0.0001) in the TCGA database and decreased with the progression of tumor staging (P<0.05); CYFIP2 gene was also expressed at a low level after the occurrence of lymph node metastasis and distal metastasis (PN<0.05, PM<0.01); the 5-year survival rate of patients was significantly higher in the group with high CYFIP2 expression than that in the group with low expression (P<0.05). The cell proliferation activity assay showed that the proliferation activity was significantly enhanced in si-CYFIP2 group than that in si-NC group (P<0.0001) and the number of cell migration was significantly increased (P<0.0001), while the proliferation activity and migration ability of OE-CYFIP2 group were significantly inhibited (P<0.001) compared with that of OE-NC group. Conclusion The CYFIP2 gene had a significant inhibitory effect on the proliferation and migratory ability of KIRC cells and may have a potential antitumor effect, which can be one of the candidate genes for the subsequent clinical diagnosis and treatment of KIRC.

renal clear cell carcinoma  /  CYFIP2  /  cell proliferation  /  cell migration
Zhe-Kun An, Xiu-Bin Li, Chen-Yu Lin, Xin-Xin Guo, Xiao-Long Cheng, Ming Cai. Role of CYFIP2 in proliferation and migration of renal clear cell carcinoma cells[J]. Medical Journal of Chinese People’s Liberation Army, 2022 , 47 (1) : 26 -32 . DOI: 10.11855/j.issn.0577-7402.2022.01.0026
Funding
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  • National Natural Science Foundation of China(81802804)
  • Incubation Program of Military Medical Science and Technology Youth Cultivation Plan(19QNP060)
  • Special Project for Cultivating "Excellent Youth" of Chinese PLA General Hospital(2020-YQPY-006)
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Year 2022 volume 47 Issue 1
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Article Info
doi: 10.11855/j.issn.0577-7402.2022.01.0026
  • Receive Date：2021-08-12
  • Online Date：2025-12-18
  • Published：2022-01-28
Article Data
Affiliations
History
  • Received：2021-08-12
  • Accepted：2021-10-29
Funding
National Natural Science Foundation of China(81802804)
Incubation Program of Military Medical Science and Technology Youth Cultivation Plan(19QNP060)
Special Project for Cultivating "Excellent Youth" of Chinese PLA General Hospital(2020-YQPY-006)
Affiliations
    1School of Basic Medical, Shanxi Medical University, Taiyuan 030001, China
    2Institute of Respiratory Sciences, the Eighth Medical Center of Chinese PLA General Hospital, Beijing 100091, China
    3Department of Urology, the Second Affiliated Hospital of School of Medicine, Zhejiang University, Hangzhou 310009, China
    4Department of Urology, the Third Medical Center of Chinese PLA General Hospital, Beijing 100143, China

Corresponding:

* Cheng Xiao-Long, E-mail: ;
Cai Ming, E-mail:
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表12种不同金属材料的力学参数
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小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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