PDF
Full
Outline
Objective To investigate the expression of FOXP3 in hormone receptor-positive breast cancer tissues and its effect on invasion and migration of MCF-7 cells. Methods Formalin-fixed, paraffin-embedded blocks from 66 hormone receptor-positive breast cancer patients who received surgery at Breast Center of the Fourth Hospital of Hebei Medical University from September 2010 to October 2011 were used in this study. The protein expression level of FOXP3 was detected by immunohistochemistry. The relationship between the FOXP3 expression levels and the clinicopathologic features was analyzed.Kaplan-Meier analysis was used to assess the overall survival rate, according to the presence or absence of FOXP3 expression. The expression levels of FOXP3 mRNA in breast cancer cell lines (MDA-MB-231, MDA-MB-453, MDA-MB-474, BR-3, and MCF-7)were detected by qRT-PCR. The MCF-7 cell line, with the highest expression level of FOXP3, was selected for the following gene silencing experiment. The si-FOXP3 transfection group (transfected with si-FOXP3), si-NC transfection group (transfected with si-NC), and blank control group (without treatment) were set. The expression of FOXP3 protein was detected by Western blotting, and the migration and invasion ability of cells was detected by scratch test and Transwell test. Results The FOXP3 exhibited a heterogeneous subcellular location in tumor cells. The positive expression ratio of FOXP3 in the nucleus was 40.90%(27/66), associated with negative lymph node metastasis, lower Ki-67 index, Luminal A typing, and TNM I stage (P<0.05); positive expression ratio of FOXP3 in the cytoplasm was 54.6%(36/66). Kaplan-Meier analysis indicated that nuclear FOXP3 expression correlated with better overall survival [96.30%(26/27) vs. 82.05%(32/39), P=0.042], but the cytoplasmic FOXP3 was not significantly associated with overall survival [88.89%(32/36) vs. 86.67%(26/30), P=0.715]. qRT-PCR results showed that the MCF-7 cell line has the highest expression level of FOXP3 mRNA in five breast cancer cell lines above (P<0.05). Thus, we performed the gene silencing experiment in the MCF-7 cell line. Compared with control group, knockdown of FOXP3 significantly enhanced the migration and invasion of MCF-7 cells (P<0.05). Conclusion In hormone receptor-positive breast cancer, the prognostic significance of FOXP3 expression is relevant to the different subcellular localization of FOXP3. Nuclear FOXP3 suggested an improved overall survival, whereas cytoplasmic FOXP3 was not significantly relevant to survival. FOXP3 may reduce the migration and invasion of MCF-7 cells, and it may be a prognostic marker for breast cancer.
PDF
121
39
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62199257
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
