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Objective To observe the effect and significance of TRIM33 gene on proliferation and migration of human gastric cancer cell SGC-7901. Methods The expression of TRIM33 in normal gastric mucosa and gastric cancer tissues and its relationship with clinicopathological features and prognosis were analyzed through TCGA and TIMER databases. Human gastric cancer cell line SGC-7901 was either untreated [negative control (NC) group] or transfected with a control vector (vector group) or transfected with vector carries si-TRIM33 (si-TRIM33 group). The downregulation of TRIM33 was validated using Western blotting.Further, the CCK-8 assay and plate clone formation assay were performed to detect cell proliferation ability. The scratch healing assay and Transwell assay were employed to test cell proliferation and migration ability. The expression of epithelial-mesenchymal transition (EMT) related proteins was tested by Western blotting. The correlation between TRIM33 expression and immune cell infiltration was analyzed through the TIMER database. Results The expression level of TRIM33 in gastric cancer was significantly higher than that in normal tissues, related to age, gender, tumor stage and lymph node metastasis (P<0.05). Cell experiment showed that SGC-7901 cells with low expression of TRIM33 showed enhanced migration, decreased EMT-related protein E-Cadherin, and significantly increased vimentin and N-Cadherin (P<0.05). TIMER database analysis showed that TRIM33 mRNA level positively correlated with the expression of CD4+ T cells, CD8+ T cells, B cells, centrioles, macrophages and natural killer cells (P<0.05), and positively correlated with M2 macrophages, CD8+ T cells, centrioles and gene markers of natural killer cells CD163, CXCL9, ITGAM, KIR3DL2 expression (P<0.05). Conclusion Downregulation of TRIM33 can promote the progression of gastric cancer, which is related to the poor prognosis of patients. The mechanism may be related to enhancing the migration characteristics of gastric cancer cells by regulating the infiltration of immune cells.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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