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Objective To study the expression and significance of extracellular signaling protein-regulated kinase 1/2(ERK1/2) signal protein and protein-degrading enzyme in cartilage and subchondral bone of osteoarthritis (OA) patients. Methods From December 2017 to December 2019, 50 knee tibial plateau samples of OA patients in the Joint Surgery Department and 10 healthy knee tibial plateau samples of patients with traumatic amputation (as control) in the Trauma Department were collected from the First Affiliated Hospital of Medical College of Shihezi University. The cartilage degeneration was observed by HE staining, and the degeneration of articular cartilage was detected by OARSI Modified Manking score. OA patients were divided into mild group and severe group according to the score. The expressions of ERK1/2, pERK1/2, MMP-3, MMP-9 and ADAMT5 protein in cartilage and subchondral bone were detected by immunohistochemical staining and Western blotting. The expressions of ERK1/2, MMP-3,MMP-9 and ADAMT5 mRNA in cartilage and subchondral bone were detected by real-time PCR (RT-qPCR), and the changes of subchondral bone microstructure were measured by micro-CT. Results HE staining showed that the cartilage became thinner, the chondrocytes arranged disorderly, and the thickness of subchondral bone increased in the mild group; the cartilage almost completely disappeared, the subchondral bone obviously exposed and deformed, and the calcified cartilage thickened in the severe group. The results of OARSI score showed that compared with control group, the OARSI scores were significantly higher[(8.14±0.56) points and (23.33±0.17) points vs. (3.53±0.11) points, P<0.05] in the mild group and severe group, and the score of the severe group was significantly higher than that of the mild group (P<0.05). Immunohistochemical staining and Western blotting results showed that compared with control group, the protein expression levels of pERK1/2, MMP-3, MMP-9 and ADAMT5 in cartilage and subchondral bone in the mild group and severe group increased (P<0.05), the mRNA expression levels of MMP-3, MMP-9 and ADAMT5 in cartilage and subchondral bone in mild group and severe group obviously increased (P<0.05), but there was no significant difference in the expression level of ERK1/2 mRNA and protein between the groups (P>0.05). Micro-CT scanning showed that the microstructure of subchondral bone in the mild and severe groups was seriously disordered with cell proliferation. Compared with control group, BV/TV, Tb.Th, Tb.N and BMD in cartilage and subchondral bone in mild group and severe group significantly increased, and Tb.Sp significantly decreased (P<0.05). Conclusions pERK1/2, MMP-3, MMP-9 and ADAMT5 are highly expressed in OA cartilage and subchondral bone, and the microstructure of subchondral bone is changed.The increased expression of pERK1/2 may be one of the reasons for the increased activity of cartilage-degrading enzymes and the changes of subchondral bone microstructure.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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