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Objective To screen key targets modulating the progression of Rickettsia heilongjiang infection using a mouse infection model by profiling the transcription expression dynamics of immune-related genes. Methods A total of 20 female C3H/HeN mice were randomized into 4 groups (5 mice/group): 0 hpi (hours post-infection), 3 hpi, 3 dpi (days post-infection), and 6 dpi. Animals were infected with 5×106 CFU Rickettsia heilongjiangensis intravenously. We harvested and extracted total RNA from spleens of animals at the indicated time points. After sequencing the total RNA, we further analyzed the sequencing results by GO annotation, R language enrichment, and KEGG visualization function analysis. Results A total of 19 930 mRNAs were specifically transcribed in the spleen post-infection. Using GO annotation and enrichment, we identified three types of immune-related genes with significant changes: first, 35 innate immune-related genes, among which the transcription of irg1 and gbp10 genes involved in the IFN-γ signaling pathway rises most significantly (P<0.05); second, 31 acquired immune-related genes, among which the transcription of gzmb gene involved in the apoptosis pathway rises most significantly (P<0.05); third, 41 inflammatory responses related genes, among which the transcription of ccl4 gene involved in cytokine and cytokine receptor signaling pathway rises most significantly (P<0.05). Conclusions The irg1 and gbp10 genes involved in the IFN-γ signaling pathway, the gzmb gene involved in the apoptosis pathway, and the ccl4 gene involved in the cytokine and cytokine receptor signaling pathway affect the pathogenesis of Rickettsia heilongjiangensis infection.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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