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Study on the development characteristics of airway Club cells in mice
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Ding Jian1, Yi-Ying Wang1, Lin Zhu1, Yuan-Yuan Li1, 2, Guang-Li Zhang2, Shi-Yi Chen1, Zheng-Xiu Luo2, *
Medical Journal of Chinese People’s Liberation Army | 2022, 47(4) : 340 - 345
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Medical Journal of Chinese People’s Liberation Army | 2022, 47(4): 340-345
• Basic Research •
Study on the development characteristics of airway Club cells in mice
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Ding Jian1, Yi-Ying Wang1, Lin Zhu1, Yuan-Yuan Li1, 2, Guang-Li Zhang2, Shi-Yi Chen1, Zheng-Xiu Luo2, *
Affiliations
  • 1Institute of Pediatrics, Children’s Hospital Affiliated to Chongqing Medical University/Key Laboratory of Child Development Disease Research, Ministry of Education/National Center for Clinical Medical Research on Child Health and Diseases/National International Scientific and Technological Cooperation Base for Major Diseases of Child Development/Chongqing Key Laboratory of Pediatrics, Chongqing 400014, China
  • 2Department of Respiratory Medicine, Children’s Hospital Affiliated to Chongqing Medical University, Chongqing 400014, China
Published: 2022-04-28 doi: 10.11855/j.issn.0577-7402.2022.04.0340
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Objective To clear the developing characteristics of airway Club cells in mice model. Methods The lung tissues and serum were collected from neonatal (1-week-old), infant (3-week-old) and adult (6-week-old) BALB/c mice. The immunofluorescence method was performed to detect the distribution and expression abundance of Club cells in main bronchi and bronchioles. The flow cytometry was used to determine the quantity proportion and proliferation ability, and ELISA was used to detect the levels of Club cell secreted protein (CC16) in lung homogenate and serum. Results The Club cells unevenly distributed in main bronchus of all ages of mice, while was relatively flat and uniform in bronchioles in BALB/c mice model. With increasing of mice age, the mean fluorescence intensity (MFI) of Club cells decreased in main bronchus accompanied by an increase in bronchioles with significant difference (main bronchus: 0.73±0.12, 0.43±0.05, 0.26±0.08, respectively, P<0.05; bronchioles:0.49±0.07, 0.73±0.08, 1.02±0.19, respectively, P<0.05). The proportion of Club cells in airway epithelium was significantly lower in neonatal (9.49%±2.38%) than those in infant (15.45%±3.86%) and adult (17.23%±4.82%) (P<0.05). Meanwhile, the proliferative ability of Ki67 in Club cells was significantly higher in neonatal (6.12%±1.89%) than those in infant (2.36%±0.98%)and adult (1.94%±0.75%) (P<0.01). The CC16 levels in lung homogenates and serum had a statistically significant increase in infant [(89.31±5.41) ng/ml, (25.77±4.68) ng/ml] and adult [(95.74±3.31) ng/ml, (28.02±3.99) ng/ml] than those in neonatal [(64.02±12.70) ng/ml, (13.91±3.36) ng/ml] (P<0.01). Conclusion Small airway is the prominent area for Club cells developing after birth, and neonatal is the critical period for Club cells proliferating and secreting CC16.

airway epithelium  /  Club cells  /  Club cell secreted protein  /  BALB/c mice
Ding Jian, Yi-Ying Wang, Lin Zhu, Yuan-Yuan Li, Guang-Li Zhang, Shi-Yi Chen, Zheng-Xiu Luo. Study on the development characteristics of airway Club cells in mice[J]. Medical Journal of Chinese People’s Liberation Army, 2022 , 47 (4) : 340 -345 . DOI: 10.11855/j.issn.0577-7402.2022.04.0340
Funding
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  • Chongqing Natural Science Foundation(cstc2020jcyj-msxmX0677)
  • Project of Science and Technology Committee of Yuzhong District, Chongqing(20200156)
  • General Basic Research Project from the Ministry of Education Key Laboratory of Child Development and Disorders(GBRP-202112)
Appendix
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Year 2022 volume 47 Issue 4
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153
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Article Info
doi: 10.11855/j.issn.0577-7402.2022.04.0340
  • Receive Date：2021-08-26
  • Online Date：2025-12-17
  • Published：2022-04-28
Article Data
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History
  • Received：2021-08-26
  • Accepted：2021-10-09
Funding
Chongqing Natural Science Foundation(cstc2020jcyj-msxmX0677)
Project of Science and Technology Committee of Yuzhong District, Chongqing(20200156)
General Basic Research Project from the Ministry of Education Key Laboratory of Child Development and Disorders(GBRP-202112)
Affiliations
    1Institute of Pediatrics, Children’s Hospital Affiliated to Chongqing Medical University/Key Laboratory of Child Development Disease Research, Ministry of Education/National Center for Clinical Medical Research on Child Health and Diseases/National International Scientific and Technological Cooperation Base for Major Diseases of Child Development/Chongqing Key Laboratory of Pediatrics, Chongqing 400014, China
    2Department of Respiratory Medicine, Children’s Hospital Affiliated to Chongqing Medical University, Chongqing 400014, China

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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
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Genus		 种数
Number of
species		 占总种数比例
Percentage of total
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鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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