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Objective To investigate the correlation between the expressions of TGFβR2 and microsatellite instability (MSI) in colorectal cancer (CRC) tissues, and the relationship between the two protein molecules and clinicopathological parameters. Methods The CRC and adjacent tissues of 184 patients with CRC pathologically diagnosed in the Second Affiliated Hospital of Kunming Medical University from January 1, 2019 to September 30, 2019 were collected, the expression of MSI was detected by capillary electrophoresis (CE), and the expressions of MMR and TGFβR2 proteins were detected by immunohistochemistry (IHC). Kappa consistency test was used to analyze the consistency of IHC and CE MSI results. The relative TGFβR2 mRNA expression was detected by quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR). Rank sum test was used to analyze the correlation between TGFβR2 gene mutation and MSI, and the relationship between TGFβR2 mRNA relative expression level and clinicopathological parameters. Results Among 184 CRC tissue samples, 152 cases (82.6%,152/184) were microsatellite instability low-frequency (MSI-L)/microsatellite stability (MSS) and 32 cases (17.4%, 32/184) were microsatellite instability high-frequency (MSI-H). The main results of MSI detected by CE and IHC were highly consistent, and the Kappa consistency coefficient was 0.922 (P<0.001). Compared with MSI-L/MSS, MSI-H mostly occurred in patients with stagesⅠ-Ⅱ colon cancer without lymph node metastasis (P<0.05). The protein and mRNA expression levels of TGFβR2 in CRC tissues were higher than those in the adjacent tissues (P<0.01). The mutation rate of TGFβR2 gene in MSI-H CRC was higher than that in MSI-L/MSS CRC [93.3%(28/30) vs. 14.9%(23/154), P<0.01]. The relative expression of TGFβR2 mRNA in MSI-H group was higher than that in MSI-L/MSS group (7.93±0.36 vs. 4.47±1.31, P<0.01), was higher in para-cancer tissues than in CRC tissues(9.38±0.82 vs. 4.76±1.63, P<0.01). The relative expressions of TGFβR2 mRNA in colon, tumors without metastasis, lymph node without metastasis and TNM Ⅰ-Ⅱ stages CRC tissues were higher (P<0.05). Conclusion The expressions of MSI and TGFβR2 are consistent and synergistic in tumor site, lymph node metastasis, and TNM stage.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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