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Objective To explore the effect and mechanism of phosphatase and tensin homolog (PTEN) on renal interstitial fibrosis in diabetic nephropathy. Methods We culture NRK52E cells in vitro, cells were randomly assigned to three groups: normal glucose group (NG), high glucose group (HG) and mannitol group (MA), Western blotting was used to detect the protein levels of PTEN, LC3, P62, collagen-Ⅰ and Ⅲ in vitro. To detect the effect of PTEN on autophagy and collagen, the NRK52E were randomly assigned to three groups: NG group, HG group and HG+pPTEN group, the autophagy changes were observed by laser confocal microscopy, and the changes of collagen were observed by fluorescence microscopy. Results Western blotting in vivo showed that, compared with NG group, the expression levels of PTEN and LC3 were decreased, but of P62, collagen-Ⅰ and Ⅲ obviously increased in HG group (P<0.01), while no obvious change of the expression of PTEN and LC3, P62, collagen-Ⅰ and Ⅲ in MA group. The confocal microscope showed that, the number of red spots and yellow spots were decreased and significant autophagy inhibition in HG group, there were no significant autophagy inhibition in other two groups. The cellular immunofluorescence staining showed that, the red fluorescence of PTEN and protein expression were decreased, the collagen fluorescence staining and protein expression were increased in HG group, there were no significant change in other two groups. Conclusions In NRK52E cells under high glucose, PTEN expression reduced significantly,autophagy was inhibited, and then aggravate renal fibrosis. Overexpression of PTEN can restore autophagy and alleviate the progression of renal interstitial fibrosis.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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