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Effect of miR-205 expression in cervical squamous cell carcinoma on cancer cell migration and invasion
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Jian-Bing Liu1, 2, Xiao-Yu Lin2, Wen-Long Li2, Wei Wang1, Wen-Hao Wang1, Xiao-Hua Cui2, Jian-Qing Hao2, Li Li2, Min Hao1, *
Medical Journal of Chinese People’s Liberation Army | 2022, 47(7) : 694 - 700
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Medical Journal of Chinese People’s Liberation Army | 2022, 47(7): 694-700
• Basic Research •
Effect of miR-205 expression in cervical squamous cell carcinoma on cancer cell migration and invasion
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Jian-Bing Liu1, 2, Xiao-Yu Lin2, Wen-Long Li2, Wei Wang1, Wen-Hao Wang1, Xiao-Hua Cui2, Jian-Qing Hao2, Li Li2, Min Hao1, *
Affiliations
  • 1Departments of Obstetrics and Gynecology, the Second Hospital of Shanxi Medical University, Taiyuan, Shanxi 030001, China
  • 2Department of Medical Cell Biology and Genetics, School of Basic Medical, Shanxi Medical University, Taiyuan, Shanxi 030001, China
Published: 2022-07-28 doi: 10.11855/j.issn.0577-7402.2022.07.0694
Outline
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Objective To identify the differentially expressed microRNA (miRNA, miR) in cervical squamous cell carcinoma (SCC) and explore the mechanism of critical miRNA on the occurrence and development of cervical cancer. Methods To identify differentially expressed miRNA, we employed bioinformatics methods to filter out miRNA under the conditions of |fold change(FC)|>2.5 and P<0.01. The RNA expressions of miR-205 and interleukin-32 (IL-32) were detected by in situ hybridization and qRT-PCR, respectively. We evaluated cell proliferation, migration, and invasion through CCK-8 and Transwell model. The protein level of matrix metalloproteinase-3 (MMP-2) and MMP-9 were quantified by Western blotting. Results Through bioinformatics analysis, we obtained 106 differentially expressed miRNA in cervical cancer, with 70 up-regulated and 36 down-regulated miRNAs. miR-205 was the most differentially expressed miRNA. We validated the overexpression of miR-205 in cervical squamous cell carcinoma by in situ hybridization and qRT-PCR (P<0.05). Overexpression of miR-205 could significantly promote the proliferation, migration, and invasion of cervical cancer cells (P<0.05). The overexpression or decreased expression of miR-205 can increase or inhibit the expression of IL-32 in cervical cancer cell lines HeLa and SiHa, and miR-205 can directly interact with the IL-32 promoter to increase the expression of target genes (P<0.05); the decreased expression of miR-205 and IL-32 inhibited the invasion of cells (P<0.05); moreover, the decreased expression of IL-32 could significantly inhibit the expression of the invasion-associated proteins MMP-2 and MMP-9 (P<0.05). Conclusion In cervical squamous cell carcinoma,miR-205 is dramatically up-regulated, potentially promoting the proliferation, migration, and invasion of cancer cells, which could further enhance the expression of MMP-2 and MMP-9 through IL-32 up-regulation, and ultimately improve the invasion ability of cervical cancer cells.

uterine cervical neoplasms  /  carcinoma, squamous cell  /  miR-205  /  cell proliferation  /  cell invasion  /  cell movement
Jian-Bing Liu, Xiao-Yu Lin, Wen-Long Li, Wei Wang, Wen-Hao Wang, Xiao-Hua Cui, Jian-Qing Hao, Li Li, Min Hao. Effect of miR-205 expression in cervical squamous cell carcinoma on cancer cell migration and invasion[J]. Medical Journal of Chinese People’s Liberation Army, 2022 , 47 (7) : 694 -700 . DOI: 10.11855/j.issn.0577-7402.2022.07.0694
Funding
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  • National Natural Science Foundation of China(81972452)
  • Shanxi Fundamental Research Program(20210302123320)
  • Key Research and Development (R and D) Projects of Shanxi Province(201803D31121)
  • Key Research and Development (R and D) Projects of Shanxi Province(201903D321152)
  • Special Public Welfare Industry Research of National Health and Family Planning Commission of China(201402010)
Appendix
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Year 2022 volume 47 Issue 7
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214
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Article Info
doi: 10.11855/j.issn.0577-7402.2022.07.0694
  • Receive Date：2021-10-26
  • Online Date：2025-12-16
  • Published：2022-07-28
Article Data
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History
  • Received：2021-10-26
  • Accepted：2022-04-29
Funding
National Natural Science Foundation of China(81972452)
Shanxi Fundamental Research Program(20210302123320)
Key Research and Development (R and D) Projects of Shanxi Province(201803D31121)
Key Research and Development (R and D) Projects of Shanxi Province(201903D321152)
Special Public Welfare Industry Research of National Health and Family Planning Commission of China(201402010)
Affiliations
    1Departments of Obstetrics and Gynecology, the Second Hospital of Shanxi Medical University, Taiyuan, Shanxi 030001, China
    2Department of Medical Cell Biology and Genetics, School of Basic Medical, Shanxi Medical University, Taiyuan, Shanxi 030001, China

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表12种不同金属材料的力学参数
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Family		 属数
Number of
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鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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