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The mechanism of artificial ulcer fibrosis of stenosis after full circumcision of esophageal mucosal dissection based on TGF-β1/Smads/ACTA2 signaling pathway: A porcine model
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Xin Zhou1, 2, Dan Ma3, Juan Fu2, Yun-Feng Wang3, Sheng-Chang Su3, Zu-Xin Tang1, 2, Xiao-Yu Li1, 2, Jie Chen2, 3, *, Zhi Li2, *
Medical Journal of Chinese People’s Liberation Army | 2022, 47(8) : 764 - 770
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Medical Journal of Chinese People’s Liberation Army | 2022, 47(8): 764-770
• Basic Research •
The mechanism of artificial ulcer fibrosis of stenosis after full circumcision of esophageal mucosal dissection based on TGF-β1/Smads/ACTA2 signaling pathway: A porcine model
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Xin Zhou1, 2, Dan Ma3, Juan Fu2, Yun-Feng Wang3, Sheng-Chang Su3, Zu-Xin Tang1, 2, Xiao-Yu Li1, 2, Jie Chen2, 3, *, Zhi Li2, *
Affiliations
  • 1School of Integrated Traditional Chinese and Western Medicine, Southwest Medical University, Luzhou, Sichuan 646000, China
  • 2Department of Spleen and Stomach Diseases, Chinese Medicine Hospital Affiliated to Southwest Medical University, Luzhou, Sichuan 646000, China
  • 3Department of Gastroenterology, the First Affiliated Hospital of Naval Medical University, Shanghai 200082, China
Published: 2022-08-28 doi: 10.11855/j.issn.0577-7402.2022.08.0764
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Objective To investigate the mechanism of artificial ulcer fibrosis of stenosis after full circumcision of esophageal mucosal dissection in porcine model based on TGF-β1/Smads/ACTA2 signaling pathway. Methods A total of eight pigs were randomized into two groups (4 in each group): sham operation group and model group. Animals in the model group received full circumcision of ESD to establish the esophageal artificial ulcer fibrosis model. Three weeks after the surgery, we collected esophageal tissues from animals. We further analyzed the tissues with Hematoxylin-eosin (HE) staining to observe the pathological characteristics of esophageal tissues. The real-time quantitative polymerase chain reaction (qRT-PCR) was employed to detect the mRNA relative expression levels of TGF-β1, Smad2, Smad3, and ACTA2. We then examined the positive expression of CTGF and ACTA2 using immunohistochemistry. Lastly, we detected the protein relative expression levels of TGF-β1, Smad2/3,p-Smad2/3, Smad4, Smad7, CTGF, and ACTA2 using Western blotting. Results Compared with the sham operation group, the fibroblasts in the artificial ulcer site proliferated rapidly with a more transformed myofibroblast phenotype in the model group. In the model group, the qRT-PCR results showed up-regulated mRNA levels of TGF-β1 (P<0.001), Smad3 (P=0.004), and ACTA2(P=0.001). The results of immunohistochemistry showed that the positive expression of CTGF (P<0.001) and ACTA2 (P<0.001)in the model group were higher than those in the sham operation group. We also observed up-regulated levels of TGF-β1 (P=0.002),Smad2/3 (P=0.003), p-Smad2/3 (P=0.002), Smad4 (P<0.001), Smad7 (P=0.016), CTGF (P<0.001), and ACTA2 in the model group, compared with the sham operation group. Conclusion A porcine model of stenosis after full circumcision of esophageal mucosal dissection was successfully established, and the mechanism may be related to the artificial ulcer fibrosis by TGF-β1/Smads/ACTA2 signaling pathway.

endoscopic submucosal dissection  /  esophageal stenosis  /  fibrosis  /  transcription growth factor β1  /  myofibroblasts
Xin Zhou, Dan Ma, Juan Fu, Yun-Feng Wang, Sheng-Chang Su, Zu-Xin Tang, Xiao-Yu Li, Jie Chen, Zhi Li. The mechanism of artificial ulcer fibrosis of stenosis after full circumcision of esophageal mucosal dissection based on TGF-β1/Smads/ACTA2 signaling pathway: A porcine model[J]. Medical Journal of Chinese People’s Liberation Army, 2022 , 47 (8) : 764 -770 . DOI: 10.11855/j.issn.0577-7402.2022.08.0764
Funding
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  • Plan Project of Science and Technology Department of Sichuan Province(2020YFS0376)
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Year 2022 volume 47 Issue 8
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Article Info
doi: 10.11855/j.issn.0577-7402.2022.08.0764
  • Receive Date：2021-11-16
  • Online Date：2025-12-15
  • Published：2022-08-28
Article Data
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History
  • Received：2021-11-16
  • Accepted：2022-02-10
Funding
Plan Project of Science and Technology Department of Sichuan Province(2020YFS0376)
Affiliations
    1School of Integrated Traditional Chinese and Western Medicine, Southwest Medical University, Luzhou, Sichuan 646000, China
    2Department of Spleen and Stomach Diseases, Chinese Medicine Hospital Affiliated to Southwest Medical University, Luzhou, Sichuan 646000, China
    3Department of Gastroenterology, the First Affiliated Hospital of Naval Medical University, Shanghai 200082, China

Corresponding:

* Li Zhi, E-mail: ;
Chen Jie, E-mail:
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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