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Effects and mechanism of RPDPC on proliferation and apoptosis of cervical cancer HeLa cells
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Feng Chen1, 2, Xiao-Hua Zeng3, Bin-Qiang Zhang3, Bin Li2, Yan-Jiao Wang2, Qin-Hua Chen3, Li-Na Ke2, *
Medical Journal of Chinese People’s Liberation Army | 2022, 47(9) : 879 - 885
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Medical Journal of Chinese People’s Liberation Army | 2022, 47(9): 879-885
• Basic Research •
Effects and mechanism of RPDPC on proliferation and apoptosis of cervical cancer HeLa cells
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Feng Chen1, 2, Xiao-Hua Zeng3, Bin-Qiang Zhang3, Bin Li2, Yan-Jiao Wang2, Qin-Hua Chen3, Li-Na Ke2, *
Affiliations
  • 1Graduate Educational Base of Sinopharm Dongfeng General Hospital, Jinzhou Medical University, Shiyan, HuBei 442008, China
  • 2Department of Obstetrics and Gynecology, Sinopharm Dongfeng General Hospital, Hubei University of Medicine, Shiyan, HuBei 442008, China
  • 3Hubei Key Laboratory of Wudang Special Chinese Medicine Research (Hubei University of Medicine), Shiyan, HuBei 442008, China
Published: 2022-09-28 doi: 10.11855/j.issn.0577-7402.2022.09.0879
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Objective To investigate the effect of Rhopaladins' analogs RPDPC on cell biological properties of human cervical cancer HeLa cells and miR-21-5P/PTEN/PI3K signaling pathway. Methods CCK-8 assay was used to detect the effects of different concentrations of RPDPC on the cell viabilities of HeLa cells treated for different time. DMSO (control), 12.5 μmol/L,25.0 μmol/L and 50.0 μmol/L RPDPC were selected to treat HeLa cells for 48 h. Hoechst 33258 staining and Annexin-FITC/PI double staining were used to detect the effect of RPDPC on HeLa cell apoptosis; The expression levels of miR-21 and mRNA of E6,E7, PTEN, PI3K, Akt were detected by qRT-PCR; The expression levels of PTEN, p-PI3K, p-PI3K, p-Akt and Akt were detected by immunoblotting (WB). Results Compared with the control group, viabilities of HeLa cells decreased significantly treated by RPDPC in different concentration and time (P<0.05); The apoptosis rate of HeLa cells tended to increase with increasing concentration of RPDPC (P<0.05); qRT-PCR results showed that compared with the control group, the expression level of miR-21-5P and mRNA of E6, E7, PI3K, Akt decreased significantly (P<0.05), the expression levels of PTEN mRNA increased significantly(P<0.05). WB results showed that compared with the control group, the expression level of PTEN protein increased significantly(P<0.05); The expression levels of p-PI3K, PI3K, p-Akt and Akt proteins decreased significantly (P<0.05). Conclusions RPDPC could inhibit the proliferation and promote the apoptosis of cervical cancer cells. The mechanism may be the inhibition of expression of E6 and E7, and may be related to mir-21/PTEN/PI3K/Akt signaling pathway.

cervical cancer  /  marine alkaloids  /  cell proliferation  /  apoptosis  /  human papilloma virus
Feng Chen, Xiao-Hua Zeng, Bin-Qiang Zhang, Bin Li, Yan-Jiao Wang, Qin-Hua Chen, Li-Na Ke. Effects and mechanism of RPDPC on proliferation and apoptosis of cervical cancer HeLa cells[J]. Medical Journal of Chinese People’s Liberation Army, 2022 , 47 (9) : 879 -885 . DOI: 10.11855/j.issn.0577-7402.2022.09.0879
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  • National Natural Science Foundation of China(81872509)
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Year 2022 volume 47 Issue 9
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Article Info
doi: 10.11855/j.issn.0577-7402.2022.09.0879
  • Receive Date：2021-10-14
  • Online Date：2025-12-15
  • Published：2022-09-28
Article Data
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History
  • Received：2021-10-14
  • Accepted：2022-03-03
Funding
National Natural Science Foundation of China(81872509)
Affiliations
    1Graduate Educational Base of Sinopharm Dongfeng General Hospital, Jinzhou Medical University, Shiyan, HuBei 442008, China
    2Department of Obstetrics and Gynecology, Sinopharm Dongfeng General Hospital, Hubei University of Medicine, Shiyan, HuBei 442008, China
    3Hubei Key Laboratory of Wudang Special Chinese Medicine Research (Hubei University of Medicine), Shiyan, HuBei 442008, China

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多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
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栓菌属 Trametes 5 2.39
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