Home Journals Articles Updates About
CN
image
收藏切换
The effect and mechanism of long non-coding RNA HCG18 on the cisplatin resistance of ovarian cancer cells
收藏切换
PDF
Chun-Lei Qi, Guo-Qing Cai, Jia Xu, Qian Guo*
Medical Journal of Chinese People’s Liberation Army | 2022, 47(11) : 1108 - 1115
Less
收藏切换
Medical Journal of Chinese People’s Liberation Army | 2022, 47(11): 1108-1115
• Basic Research •
The effect and mechanism of long non-coding RNA HCG18 on the cisplatin resistance of ovarian cancer cells
Full
Chun-Lei Qi, Guo-Qing Cai, Jia Xu, Qian Guo*
Affiliations
  • Department of Gynaecology and Obstetrics, the First Affiliated Hospital of Air Force Medical University, Xi’an, Shaanxi 710032, China
Published: 2022-11-28 doi: 10.11855/j.issn.0577-7402.2022.11.1108
Outline
收藏切换

Objective To explore the effects and mechanism of long non-coding RNA HLA complex group 18 (HCG18)on human ovarian cancer cell SKOV3 and its cisplatin resistance cell line SKOV3/DDP. Methods RT-PCR was used to detect the expression levels of HCG18 and miR-449 in SKOV3 and SKOV3/DDP cells. Cells were transfected with HCG18 siRNA and treated with DDP (12.5 μg/ml). After 48 h, we evaluated cell viability using the MTT assay, examined cell apoptosis and cell cycle through flow cytometry, and confirmed targeting binding of HCG18, miR-449, and Wnt1 with luciferase reporter assay. Results Compared with the human normal ovarian epithelial cell line NOEC, the HCG18 level was significantly increased (P<0.05), and the miR-449 level was significantly decreased in SKOV3 (P<0.05). Compared with the transfection control group, in the DDP-treated cells (both SKOV3 and SKOV3/DDP), we observed significantly reduced cell viability, enhanced cell apoptosis, and cell cycle G2/M phase arrest (P<0.05). Transfection of MiR-449 inhibitor partly reversed the effects of HCG18 siRNA on cell viability, cell apoptosis, and cell cycle in both SKOV3 and SKOV3/DDP cells (P<0.05). The luciferase reporter results showed that HCG18 could bind to the 3'-UTR of miR-449, and miR-449 could bind to the 3'-UTR of Wnt1. Transfection of HCG18 siRNA significantly increased the levels of miR-449 (P<0.05). Transfection of HCG18 siRNA or miR-449 mimic significantly decreased the mRNA levels of Wnt1(P<0.05). Conclusions HCG18 knockdown could inhibit cell viability, promote cell apoptosis and cell cycle G2/M phase arrest through miR-449/Wnt1 signaling pathway, and thus promote cisplatin sensitivity of human ovarian cancer cells.

ovarian cancer  /  lncRNA HCG18  /  microRNA-449  /  genes, Wnt1
Chun-Lei Qi, Guo-Qing Cai, Jia Xu, Qian Guo. The effect and mechanism of long non-coding RNA HCG18 on the cisplatin resistance of ovarian cancer cells[J]. Medical Journal of Chinese People’s Liberation Army, 2022 , 47 (11) : 1108 -1115 . DOI: 10.11855/j.issn.0577-7402.2022.11.1108
Funding
Less
  • Key Research and Development Plan of Shaanxi Province(2019SF-165)
Appendix
Less
Year 2022 volume 47 Issue 11
PDF
243
110
Cite this Article
BibTeX
Article Info
doi: 10.11855/j.issn.0577-7402.2022.11.1108
  • Receive Date：2021-06-21
  • Online Date：2025-12-14
  • Published：2022-11-28
Article Data
Affiliations
History
  • Received：2021-06-21
  • Accepted：2021-07-28
Funding
Key Research and Development Plan of Shaanxi Province(2019SF-165)
Affiliations
    Department of Gynaecology and Obstetrics, the First Affiliated Hospital of Air Force Medical University, Xi’an, Shaanxi 710032, China

Corresponding:

References
Share
https://castjournals.cast.org.cn/joweb/jfjyxzz/EN/10.11855/j.issn.0577-7402.2022.11.1108
Share to
QR

Scan QR to access full text

Cite this article
BibTeX
Citations
表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
关闭全屏
  • BibTeX
  • EndNote
  • RefWorks
  • TxT
Links
Articles
Latest Articles
Most Read
Collections
Updates
Events
News
Multimedia
About
About Us
Contact
No. 86 Xueyuan South Road, Haidian District, Beijing
100081
010-62199257
qkjq@cast.org.cn

Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House