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Objective To investigate the effect and mechanism of Paris saponins on iron death in triple negative breast cancer cells. Methods MDA-MB-231 cells were treated with 0, 10, 20, 30, 40 and 50 μmol/L of Paris saponins. Cell viability was detected by CCK-8 method. MDA-MB-231 cells at logarithmic growth stage were randomly divided into control group, Paris saponin group (treated with 30 μmol/L Paris saponin for 12 h), inhibitor group (treated with 2 μmol/L Ferrostatin for 12 h) and Paris saponin + inhibitor group (treated with 30 μmol/L Paris saponin for 12 h after treatment with 2 μmol/L Ferrostatin-1 for 12 h), cell viability was detected by CCK-8 method, cell apoptosis rate was detected by flow cytometry. The expressions of divalent metal ion transporter 1 (DMT1), transferrin receptor 1 (TFR1), glutathione peroxidase 4 (GPX4), p53 and p53/SLC7A11 were detected by qRT-PCR and Western blotting. Results CCK-8 assay showed that the survival rate of MDA-MB-231 cells was decreased with the increase of the concentration of Paris saponin in a dose-dependent manner (P<0.05). Compared with control group, the cell survival rate, the relative expression levels of CPX4, SLC7A11 mRNA and protein decreased, while of apoptosis rate, the relative, expression levels of TFR1, DMT1, p53 mRNA and protein increased in Paris saponin group, the relative expression levels of CPX4, SLC7A11 mRNA and protein increased in inhibitor group, the apoptosis rate and the relative expressions of TFR1, DMT1 and p53 mRNA and protein were decreased (P<0.05). Compared with inhibitor group, the cell survival rate and the mRNA and protein relative expressions of CPX4 and SLC7A11 decreased, while the apoptosis rate and the mRNA and protein relative expressions of TFR1, DMT1 and p53 increased in parisaponin + inhibitor group (P<0.05). Conclusion Paris saponin inhibits the proliferation of triple negative breast cancer cells and promotes iron death in cancer cells, possibly through the regulation of p53/SLC7A11 signal axis.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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