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Bisphenol A interfere with maturation of mouse oocytes in vitro through oxidative stress
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Liao Wan1, Shuang Wang2, Jin-Hong Yu3, Jing-Ru Ding3, Qing Zou4, Xi-Yan Wang2, *
Medical Journal of Chinese People’s Liberation Army | 2023, 48(2) : 198 - 203
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Medical Journal of Chinese People’s Liberation Army | 2023, 48(2): 198-203
• Basic Research •
Bisphenol A interfere with maturation of mouse oocytes in vitro through oxidative stress
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Liao Wan1, Shuang Wang2, Jin-Hong Yu3, Jing-Ru Ding3, Qing Zou4, Xi-Yan Wang2, *
Affiliations
  • 1School of Public Health, Jilin Medical University, Jilin City, Jilin 132013, China
  • 2Morphology Laboratory, Jilin Medical University, Jilin City, Jilin 132013, China
  • 3Clinical Medical College, Jilin Medical University, Jilin City, Jilin 132013, China
  • 4College of Pharmacy, Jilin Medical University, Jilin City, Jilin 132013, China
Published: 2023-02-28 doi: 10.11855/j.issn.0577-7402.2023.02.0198
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Objective To investigate the effect of exposure to bisphenol A (BPA) on maturation and oxidative stress injury in mouse oocytes in vitro. Methods Oocytes from female ICR mice were randomly divided into control group, DMSO group,and BPA group. Mouse oocytes in control group was cultured normorly in vitro,while added 0.1% DMSO in DMSO group, and 45 μmol/L BPA in 0.1% DMSO in BPA group. MII oocytes and oocytes stagnated in the GV phase were obtained .The levels of ROS in MII and GV oocytes of each group was detected by kit. The expression level of antioxidant enzyme in MII and GV oocytes was detected by immunofluorescence and Western blotting. Results Compared with control group, exposure to 45 μmol/L BPA during in vitro maturation, resulted in a significant decrease in the maturation rate of mouse oocytes in BPA group (26.32%±1.12%vs. 98.22%±0.89%, P<0.05), the levels of ROS in GV and MII oocytes were significantly higher (P<0.05), the levels of antioxidant enzymes CAT and SOD2 in GV and MII oocytes were significantly decreased (P<0.05). Conclusion Exposure to 45 μmol/L BPA increased ROS production and inhibited antioxidant enzyme expression, leading to oxidative stress damage of mouse oocytes and thus inhibiting oocyte maturation in vitro.

bisphenol A  /  oocytes  /  oxidative stress injury
万辽, 王爽, 于金红, 丁婧如, 邹清, 王喜艳. 双酚A通过氧化应激干扰小鼠卵母细胞体外成熟. 解放军医学杂志, 2023 , 48 (2) : 198 -203 . DOI: 10.11855/j.issn.0577-7402.2023.02.0198
Liao Wan, Shuang Wang, Jin-Hong Yu, Jing-Ru Ding, Qing Zou, Xi-Yan Wang. Bisphenol A interfere with maturation of mouse oocytes in vitro through oxidative stress[J]. Medical Journal of Chinese People’s Liberation Army, 2023 , 48 (2) : 198 -203 . DOI: 10.11855/j.issn.0577-7402.2023.02.0198
Funding
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  • Innovation and Entrepreneurship Training Programme of Jilin Province for College Students(201913706069)
Appendix
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Year 2023 volume 48 Issue 2
675
321
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Article Info
doi: 10.11855/j.issn.0577-7402.2023.02.0198
  • Receive Date：2021-09-17
  • Online Date：2025-12-03
  • Published：2023-02-28
Article Data
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History
  • Received：2021-09-17
  • Accepted：2021-11-19
Funding
Innovation and Entrepreneurship Training Programme of Jilin Province for College Students(201913706069)
Affiliations
    1School of Public Health, Jilin Medical University, Jilin City, Jilin 132013, China
    2Morphology Laboratory, Jilin Medical University, Jilin City, Jilin 132013, China
    3Clinical Medical College, Jilin Medical University, Jilin City, Jilin 132013, China
    4College of Pharmacy, Jilin Medical University, Jilin City, Jilin 132013, China

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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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