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Objective To explore the differences in the intestinal flora between the glucocorticoid-associated osteonecrosis of the femoral head (GA-ONFH) rat model and its effect on the modeling results of GA-ONFH. Methods Forty SD rats were randomly divided into four groups (n=10 for each group): control group, antibiotic (ABX) group, GA-ONFH model group, and ABX+GA-ONFH group. The ABX group and ABX+GA-ONFH group were continuously treated with an antibiotic solution to elute intestinal microorganisms for one week. One week later, the GA-ONFH model group and ABX+GA-ONFH group were intraperitoneally injected with lipopolysaccharide and dexamethasone to create an early GA-ONFH model; while the control group and ABX group received an equal volume of normal saline for six weeks. After six weeks, to evaluate correlation between intestinal flora and GA-ONFH modeling, the following experiments were conducted and analyzed from intestinal contents and femur samples: 1) the composition and abundance of fecal flora based on 16S rDNA amplicon sequencing technology; 2) Micro-CT and HE staining to observe the modeling results of early GA-ONFH. Results Compared with control group, the abundance of intestinal flora in GA-ONFH model group changed, and the abundance of Bacteroidetes was significantly down-regulated and Firmicutes was significantly up-regulated, with statistical significance (P<0.05); at the family level, the abundance of Prevotellaceae, Clostridia-UCG-014, Peptococcaceae, Rikenellaceae, and Christensenellaceae were significantly down-regulated, the abundance of Staphylococcaceae was significantly up-regulated, and the differences were statistically significant (P<0.05); at the genus level, the abundance of Alistipes, UCG-005, Clostridia_UCG-014, Alloprevotella, and Prevotellaceae_UCG-001 were significantly down-regulated, the abundance of Staphylococcus and Frisingicoccus were significantly up-regulated, with statistical significance (P<0.05).Micro-CT tomography showed subchondral trabecular sclerosis in both the GA-ONFH model group and ABX+GA-ONFH group after modeling. HE staining showed that trabecular bone fracture occurred earlier in the ABX+GA-ONFH group than in the GA-ONFH model group. After the gut microbial abundance was removed, the rat GA-ONFH modeling effect was more significant. Conclusion The intestinal flora of GA-ONFH model rats changed, and the change of intestinal flora may affect the occurrence of early GA-ONFH.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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