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Objective To observe the changes in depression-like behavior and cAMP-PKA-CREB-BDNF signaling pathway in the hippocampus of rats with mild brain blast injury (mbTBI) caused by blast shock wave, and to explore the mechanism of neurological dysfunction caused by mbTBI. Methods 44 male SD rats were randomly divided into control group and model group, 22 in each group. The rats in the model group were placed in a gridded iron cage, with the head facing the explosion center and fixed on the ground 15 m away from the explosion center in a fan shape. At the same time, the shock wave intensity at the location of the rats was detected. After the explosion, 7 rats in each group were randomly selected and fed in separate cages. Open field test and elevated plus maze test were used to evaluate the depression-like behavior of rats at 7, 14 and 28 d after exposure. The remaining rats in each group were randomly sacrificed at 7, 14 and 28 d after exposure. The hippocampus was removed and protein extracted. Western blotting was used to detect the relative expression of cyclic adenosine monophosphate (cAMP), protein kinase A (PKA), cyclic adenosine monophosphate response element binding protein (CREB), phosphorylated CREB (p-CREB), brain-derived neurotrophic factor (BDNF) and oncogenic protein c-FOS. Results After the explosion test, all the rats in the model group survived, but within 24 hours, they were apathetic, slow in action and poor in appetite. After 72 hours, the above symptoms gradually eased. There was no significant difference in body weight between the two groups at each time point (P>0.05). The results of behavioral experiments showed that compared with the control group, the total moving distance, the residence time in the central area, the number of times and the proportion of time entering the open arm of the model group were significantly reduced at 14 d after the explosion test (P<0.05). The results of Western blotting showed that compared with the control group, the expression levels of PKA, CREB, pCREB and BDNF protein in the model group were significantly decreased at 7, 14 and 28 d after explosion test (P<0.05). Compared with the control group, the expression of cAMP and c-FOS in the model group was significantly decreased at 7 and 14 d after the explosion test (P<0.05), and there was no significant difference between the two groups at 28 d(P>0.05). Compared with 7 and 14 d after the explosion test, the expression levels of PKA, CREB and BDNF in the model group were significantly increased at 28 d after the test (P<0.05). Conclusion Single blast shock wave exposure can cause depression-like behavior changes in rats, which may be related to the down-regulation of the cAMP-PKA-CREB-BDNF signaling pathway.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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