PDF
Full
Outline
Objective To evaluate the hemostatic efficacy and safety of a new type of pushable compressed cellulose hemostatic device in emergency treatment of deep tissue massive hemorrhage. Methods The internal compressed cellulose hemostatic granules were placed in excess normal saline to test their saline absorption and volume expansion properties. Femoral artery blood was taken from 3 big-eared white rabbits for measuring the in vitro clotting time of the compressed cellulose hemostatic granules, and equal quality CELOX-A® hemostatic powder was set as the positive control group, and the blank plasma as negative control group. Ten rats were divided into the compressed cellulose hemostatic granule dressing group, CELOX-A® hemostatic powder group and negative control group, and plasma was taken to test the coagulation activity by using a thromboelastometer. The swine models of massive hemorrhage were established by complete transection of the femoral artery and vein, and divided into the model group of medical defatted gauze block, the pushable compressed cellulose hemostatic device group, and the positive control group of CELOX-A®. The three groups used parallel operation for hemostasis. The number of pushable compressed cellulose hemostatic device used in the hemostasis process, the number of presses, the hemostasis time, the total blood loss during the hemostasis process were recorded, the routine blood tests before and after hemostasis, the prothrombin time (PT) and activated partial thromboplastin time (APTT) were measured and HE staining was used to observe the pathological changes of the tissue vessels. After DMEM medium was used to extract the pushable compressed cellulose hemostatic device, and the extract was added to L929 cells with vigorous growth to test the cytotoxicity. At the same time, the complete DMEM medium with phenol was set as the positive control group and the complete DMEM medium as the negative control group. After pushable compressed cellulose hemostatic device was extracted with normal saline, the normal saline extract was injected into the mice to test its acute toxicity, and equal volume of the normal saline into the mice in the same way as the negative control group. The normal saline was set as the negative control group and the deionized water was set as the positive control group, the hemolysis test was used to detect the hemolysis rate of pushable compressed cellulose hemostatic device. Results The compressed cellulose hemostatic granules inside the device rapidly absorbed and expanded in normal saline buffer, the maximum absorption ratio was more than 7.3 times, and the swelling rate reached about (9.0±0.3) times after 3 s in normal saline buffer. The in vitro clotting time of the compressed cellulose hemostatic granules was (451.7±26.6) s, significantly shorter than that of the negative control group (703.7±32.1) s (P<0.01), and was also shorter than that of the CELOX-A® hemostatic powder about (521.7±18.1) s (P<0.05). The result of thromboelastography showed that compared with negative control group, both the compressed cellulose hemostatic granules and CELOX-A® hemostatic powder significantly shortened the clot formation time (P<0.01), and the compressed cellulose hemostatic granules had shorter coagulation time than CELOX-A® hemostatic powder (P<0.05). The evaluation results of hemostatic efficacy study on the model of massive groin hemorrhage by complete transaction of the femoral artery and vein in the swine showed the pushable compressed cellulose hemostatic device group used (1.2±0.4) devices to achieve hemostasis, while CELOX-A® group needed (2.2±0.4) devices (P<0.05), and the total hemostatic time needed for the pushable compressed cellulose hemostatic device and CELOX-A® was (185.5±2.4) s and (268.5±83.4) s, respectively, and the difference between them was statistically significant (P<0.05). The APTT, PT and pathological changes of tissue and blood vessels were not obvious after the experiment in the pushable compressed cellulose hemostatic device group. The results of in vitro cytotoxicity test, acute toxicity test and hemolysis test were all within the standard range. Conclusions Pushable compressed cellulose hemostatic device has favourable hemostatic efficacy and biological safety. The compressed cellulose hemostatic granule can rapidly block the injured vessel and effectively fill the wound cavity based on its strong absorption and swelling property. To summarize, the pushable compressed cellulose hemostatic device is a promising candidate to be used for controlling junctional hemorrhage and evacuation of the wounded, especially for the hemorrhage caused by gunshot or penetrating injuries in battlefield, thus saving valuable time for the further evacuation and treatment.
PDF
199
95
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62199257
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
