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Objective To analyze the level of reactive oxygen species (ROS) produced by neutrophils and to explore its clinical significance in patients with chronic hepatitis B virus (HBV) infection. Methods Eighty-eight chronic HBV infected patients who admitted to the Fifth Medical Center of Chinese PLA General Hospital from April 2022 to September 2022 were enrolled in this study. Thirty patients who received nucleoside (acid) analogues (NAs) treatment were classified as the treated group, and fifty-eight did not receive treatment were classified as the untreated group. Simultaneously, twenty healthy individuals were classified as control group. The differences in clinical and virological parameters were compared between the three groups. The spontaneous ROS production by neutrophils after incubation with DHR 123 fluorescent dye were detected by flow cytometry. And the correlation between the spontaneous ROS and HBV DNA viral load, hepatitis B surface antigen (HBsAg), white blood cell (WBC), C-reactive protein (CRP), alanine transaminase (ALT) and aspartate transaminase (AST) were further analyzed. Culture medium, HepG2 cell supernatant and HepG2.2.15 cell supernatant were used to co-culture with neutrophils from healthy controls, respectively, to verify the effect of HBV virus particles and proteins on neutrophil spontaneous ROS in vitro. The secondary ROS production by neutrophils after lipopolysaccharide (LPS) stimulation was compared between the three groups. Results The spontaneous ROS production by neutrophils in the untreated group was higher than control group (P<0.05). The spontaneous ROS production by neutrophils in untreated patients was positively correlated with HBV DNA viral load and HBsAg quantification (r=0.315, P=0.016; r=0.326, P=0.013), but had no significant correlation with WBC, CRP, ALT and AST. HepG2.2.15 cell supernatant could induce the spontaneous ROS production by neutrophils in a dose-dependent manner (P<0.05). After LPS stimulation, the secondary ROS production by neutrophils in the untreated group and the treated group were lower than the control group (P<0.05). Conclusion Reducing the viral load by antiviral treatment contributes to reduction of spontaneous ROS production by neutrophils but not to restore the secondary ROS production by neutrophils after stimulated by LPS.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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