PDF
Full
Outline
Objective To examine the differences between mouse models of classic heat stroke (CHS) with multiple organ dysfunction via two different rising strategies. Methods A total of 66 male C57BL/6J mice were divided into direct heat stroke (DHS) group (n=28), a stepwise heat stroke (SHS) group (n=28), and control group (n=10) using the random number table method. In the first two groups, animals received direct warming at 41 ℃ and stepwise warming from 25.0 ℃ to 39.5 ℃, using a simulated climate chamber, respectively. While the animals were in the climate chamber before reaching the endpoint, we constantly monitored the animal activity, animal consciousness, and rectal temperature. We randomly selected 4 animals from each group and collected the blood samples and organ tissues (liver, kidney, intestine, lung, and spleen) after 24 hours of recovery since the end of heat exposure. We used the automatic biochemical analyzer to measure the levels of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), creatinine (CREA), blood urea nitrogen (BUN), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), and creatine kinase isoenzyme MB (CK-MB). We employed multifactorial test kits to detect the levels of interleukin (IL)-1β, IL-6, tumor necrosis factor (TNF)-α, monocyte chemoattractant protein (MCP)-1 and transforming growth factor (TGF)-β. We analyzed the histological sections from each organ and then calculated the pathological injury scores. We saved the remaining mice for the 72 h survival analysis. Results Both heat stroke strategies can establish a stable CHS model of the mouse. In contrast with mice in DHS group, mice in SHS group exposed to heat for a longer time [(181.61±41.88) min vs. (104.72±18.68) min, P<0.001], had a higher percentage of dehydration [(11.59±1.52)% vs. (7.07±1.84)%, P<0.001] and higher 72 h mortality (73.68% vs. 22.22%, P<0.05). After 24 hours' recovery, biochemical indicators (ALT, AST, CREA, BUN) of SHS group were higher than those of DHS group [(875.63±241.24) U/L vs. (139.38±188.22) U/L, P<0.01; (2406.75±1008.69) U/L vs. (208.13±149.23) U/L, P<0.01; (79.88±41.39) U/L vs. (18.75±10.51) U/L, P<0.05; (134.33±52.54) U/L vs. (17.75±7.31) U/L, P<0.01]. The pathological injury scores of each organ tissue of the SHS group were higher than those of the DHS group (P<0.05). The levels of MCP-1 of the SHS group were higher than that of the DHS group [(22.89±1.97) pg/ml vs. (15.97±3.91) pg/ml, P<0.05], and TGF-β of SHS group was lower than that of DHS group [(936.46±30.17) pg/ml vs. (1453.50±129.81) pg/ml, P<0.001]. Conclusions The stepwise warming method had a higher success rate in developing the model, a higher mortality rate within 72 h, and more severe organ damage than the direct warming method. Thus it was a more stable and reliable modeling method that was more consistent with the pathophysiological state of CHS patients at the actual onset of illness.
PDF
342
160
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62199257
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
