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Prediction of T cell epitopes of Hantavirus glycoprotein Gn and cross species validation
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Bao-Zeng Sun1, Cong-Cong Qin1, Jun-Qi Zhang1, Yong-Kai Wang1, Wen-Biao Zhang1, Rui-Bo Liu1, Tian-Yuan Bai1, Zhi-Hui Zhang1, Yu-Si Zhang1, Kun Yang1, 2, 3, Dong-Bo Jiang1, 2, 4, *
Medical Journal of Chinese People’s Liberation Army | 2023, 48(9) : 1000 - 1010
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Medical Journal of Chinese People’s Liberation Army | 2023, 48(9): 1000-1010
• Basic Research •
Prediction of T cell epitopes of Hantavirus glycoprotein Gn and cross species validation
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Bao-Zeng Sun1, Cong-Cong Qin1, Jun-Qi Zhang1, Yong-Kai Wang1, Wen-Biao Zhang1, Rui-Bo Liu1, Tian-Yuan Bai1, Zhi-Hui Zhang1, Yu-Si Zhang1, Kun Yang1, 2, 3, Dong-Bo Jiang1, 2, 4, *
Affiliations
  • 1Department of Immunology, Basic Medicine School, Air Force Medical University, Xi'an, Shaanxi 710032, China
  • 2Key Laboratory of Biological Weapon Damage and Prevention Drugs, Basic Medicine School, Air Force Medical University, Xi'an, Shaanxi 710032, China
  • 3Department of Rheumatology, Tangdu Hospital, Air Force Medical University, Xi'an, Shaanxi 710032, China
  • 4Department of Microbiology, Basic Medicine School, Air Force Medical University, Xi'an, Shaanxi 710032, China
Published: 2023-09-28 doi: 10.11855/j.issn.0577-7402.1489.2023.0419
Outline
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Objective To screen, identify and validate the immunoreactive epitopes on the glycoprotein-N terminal (Gn) of Hantaan virus (HTNV) for providing a new idea for prevention of hemorrhagic fever with renal syndrome (HFRS). Methods The Gn protein sequences of HTNV strain 76-118 were obtained from UniProt database. IEDB, SMMPMBEC, NetMHCpan 4.1, SYFPEITHI and Rankpep were used to predict epitope affinity. Immunogenicity was analyzed by VaxiJen. Blastp analyzed the conservation; HPEPDOCK and EpiDOCK simulated pMHC docking; TBtools implemented bidirectional cluster analysis; Immunoreactivity of epitope in vivo was evaluated by enzyme-linked immunospot assay. Results The Gn protein sequence of HTNV 76-118 strain (PRO_0000036816) was obtained from UniProt database. Five affinity algorithms were integrated to obtain 61 dominant epitopes in mouse H-2 subtype, 234 dominant epitopes in human major histocompatibility complex (MHC)-Ⅰ subtype, and 212 dominant epitopes in MHC-Ⅱ subtype. VaxiJen screening obtained 23, 110 and 42 dominant epitopes of H-2, MHC-Ⅰ and MHC-Ⅱ subtypes, respectively. Further Blastp screening resulted in 3 MHC-Ⅰ restricted epitopes with high affinity and strong immunogenicity, and 81 MHC-Ⅱ restricted epitopes conserved between species. Bidirectional hierarchical cluster analysis revealed the similarity of HTNV Gn epitopes in H2-d, H2-b of mice and some human leucocyte antigen (HLA). ELISpot verified that 5 epitopes could induce splenic cells to secrete interferon-gamma (IFN-γ). Conclusions The present study predicted and verified the cellular immunoreactive epitopes on HTNV Gn that can induce cellular immune reactivity, revealed the cross activity across-genes, species and genera immunoreactivity of viral antigens in MHC presentation, and provide guidance for the development of novel HFRS epitope vaccines.

Hantaan virus  /  glycoprotein  /  epitope  /  in silico prediction  /  immunoreactivity
Bao-Zeng Sun, Cong-Cong Qin, Jun-Qi Zhang, Yong-Kai Wang, Wen-Biao Zhang, Rui-Bo Liu, Tian-Yuan Bai, Zhi-Hui Zhang, Yu-Si Zhang, Kun Yang, Dong-Bo Jiang. Prediction of T cell epitopes of Hantavirus glycoprotein Gn and cross species validation[J]. Medical Journal of Chinese People’s Liberation Army, 2023 , 48 (9) : 1000 -1010 . DOI: 10.11855/j.issn.0577-7402.1489.2023.0419
Funding
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  • General Program of National Natural Science Foundation of China(82073154)
  • Youth Program of National Natural Science Foundation of China(82203510)
  • Promotion Program of Air Force Medical University(2020XXXX03)
Appendix
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Year 2023 volume 48 Issue 9
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Article Info
doi: 10.11855/j.issn.0577-7402.1489.2023.0419
  • Receive Date：2022-07-12
  • Online Date：2025-11-25
  • Published：2023-09-28
Article Data
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History
  • Received：2022-07-12
  • Accepted：2022-11-05
Funding
General Program of National Natural Science Foundation of China(82073154)
Youth Program of National Natural Science Foundation of China(82203510)
Promotion Program of Air Force Medical University(2020XXXX03)
Affiliations
    1Department of Immunology, Basic Medicine School, Air Force Medical University, Xi'an, Shaanxi 710032, China
    2Key Laboratory of Biological Weapon Damage and Prevention Drugs, Basic Medicine School, Air Force Medical University, Xi'an, Shaanxi 710032, China
    3Department of Rheumatology, Tangdu Hospital, Air Force Medical University, Xi'an, Shaanxi 710032, China
    4Department of Microbiology, Basic Medicine School, Air Force Medical University, Xi'an, Shaanxi 710032, China

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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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