PDF
Full
Outline
Objective To observe the influence of exosomal miR-155 derived from monocytes stimulated by heat stress on the inflammatory response of hepatocytes. Methods According to different treatments, we termed THP-1 monocytes into the control group, the heat stress group, and the ulinasatin group. We then extracted the exosomes from each group. To study the function of exsome on hepatocytes, we incubated hepatocytes with these exosomes. We then tested the alanine aminotransferase and lactate dehydrogenase levels in the supernatant, measured cell viability, and detected the relative mRNA levels of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in the hepatocytes. Then, the expression changes of exosomal miR-155 derived from monocytes were detected using RT-qPCR. Furthermore, the potential target of miR-155 was analyzed using database retrieval, dual-luciferase report, and Western blotting assay. Finally, the effect of monocyte exosomes on hepatocyte miR-155 and the treatment with ulinastatin or miR-155 inhibitor on hepatocyte injury were observed. Results When incubating hepatocytes with the exosomes derived from monocytes stimulated by heat stress, the levels of alanine aminotransferase and lactate dehydrogenase in the supernatant and the mRNA levels of TNF-α and IL-6 in hepatocytes were all significantly increased (P<0.01) with decreased cell survival rate (P<0.05). Heat stress increased the level of miR-155 contained in monocytes and their release of exosomes. Database retrieval, dual-luciferase reports, and Western blotting assay showed that miR-155 might target SOCS1. The exosomes from monocytes stimulated by heat stress increased miR-155 in liver cells. Ulinastatin or miR-155 inhibitor could reduce the exosomal miR-155 level and decrease TNF-α and IL-6 mRNA expression in liver cells. The difference was statistically significant (P<0.05). Conclusion The monocytes stimulated by heat stress increase the inflammatory response of hepatocytes, which may be associated with increased exosomal miR-155 using SOCS1 as a potential target. Ulinastatin may relieve the hepatocytic mRNA expression of inflammatory cytokines in this setting.
PDF
199
94
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62199257
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
