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Objective To explore the effect and mechanism of acid-sensitive ion channel 3 (ASIC3) expression on visceral sensitivity in irritable bowel syndrome (IBS) rats and the possible regulatory mechanisms of signalling pathways. Methods From the 2nd day postpartum, 6 neonatal rats and their mother were kept in their original cages as control group; the rest of the neonatal rats were separated from their mother to make IBS models. After the neonatal rats grow into rats, use abdominal withdrawal reflex (AWR) score to screen 16 rats that were successful in IBS modeling, and they were randomly divided into neonatal maternal separation (NMS) group and NMS+APETx2 group, with 8 rats in each group. NMS+APETx2 group was given the ASIC3-specific blocker APETx2 100 μg/kg intraperitoneally for 1 week while NMS group and control group were intraperitoneally injected with same amount of normal saline. Visceral pain response was measured by abdominal withdraw reflex (AWR) score, the intestinal transmission rate was measured by ink staining, the 5-hydroxytrgptamine (5-HT) level in colonic tissue was measured by enzyme-linked immunosorbent assay (ELISA), and the expression levels of 5-HT4 receptor and ASIC3 in colonic tissue were measured by immunohistochemistry. Results Compared with control group, rats in NMS group showed significantly increased AWR scores at colorectal pressures of 40, 60, 80 mmHg, decreased intestinal transmission rate, intestinal 5-HT, ASIC3 and 5-HT4 receptor expression increased, all with statistically significant differences (P<0.01). There was no significant difference in AWR score and intestinal propulsion rate in NMS+APETx2 group compared with control group (P>0.05), but the expression levels of 5-HT, ASIC3 and 5-HT4 receptors increased compared with control group with significant difference (P<0.01). Compared with NMS group, the application of ASIC3-specific blocker APETx2 in NMS+APETx2 group resulted in lower AWR scores, enhanced intestinal transmission rate, and the expression levels of 5-HT, ASIC3 and 5-HT4 receptor decreased, and the differences were statistically significant (P<0.05 or P<0.01). Conclusion ASIC3 expression takes part in the generation of high sensitivity in viscera of IBS rat, which may be related to the enhance of 5-HT pathway activation.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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