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Objective To investigate the possible mechanism of Xuebijing injection in regulating abnormal degradation of pulmonary vascular endothelial calyx to improve acute lung injury induced by severe heatstroke. Methods Forty-eight Wistar rats were randomly divided into control group, heatstroke group and Xuebijing group. Before heatstroke induction, rats in Xuebijing group were administrated with Xuebijing injection (2 ml/kg, 2 times/d) for 3 days. All rats were exposed to an environment with temperature of (40±2) ℃ and humidity of 65%±5% for 60 minutes to induce heatstroke. Two hours later, the lung wet/dry weight ratio was recorded; the concentration of proteins in BALF was measured; the pulmonary vascular permeability was measured by Evans blue (EB); HE staining was used to observe the pathological changes of lung tissue; the changes of hyaluronic acid (HA) on the surface of pulmonary vessels were observed by immunofluorescence; Western blotting was used to detect the expression of Syndecan-1, Glypican-1, VE-Cadherin, Occludin, VCAM-1 and E-selectin in lung tissues; Enzyme-linked immunosorbent assay (ELISA) was utilized to quantify the concentration of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) in serum and heparanase (HPA) in lung tissue. Results Xuebijing could decrease the lung wet/dry weight ratio, reduce protein exudation and improve pulmonary vascular permeability (P<0.01); reduce the histological injury (P<0.01); reduce the degradation of HA, Syndecan-1 and Glypican-1 on the surface of pulmonary vessels (P<0.01); increase the expression of VE-Cadherin and Occludin (P<0.01); regulate the overexpression of VCAM-1 and E-selectin (P<0.01); down-regulate the expression of TNF-α, IL-6 and HPA (P<0.01). Conclusion Xuebijing injection decrease the expression of HPA, improve the disintegration of pulmonary vascular endothelial calyx, repair the integrity of pulmonary vessels, reduce the damage of cell connections, down-regulate the expression of adhesion molecules, inhibit the inflammatory reaction, relieving acute lung injury caused by severe heat stroke.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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