Objective To analyze the correlation between irritable bowel syndrome (IBS) as well as its subtypes and gallbladder stone. Methods Collected the clinical data of 556 patients who were treated in Department of Gastroenterology of the Sixth Medical Center of Chinese PLA General Hospital from January 2019 to March 2023. The patients were divided into IBS group (n=161) and non-IBS group (n=395). The subjects were investigated by questionnaire, physical examination and blood examination, and the data of gender, age, height, weight, blood pressure and blood biochemical indexes were obtained and compared between two groups. The relation between gallbladder stone and IBS were evaluated by logistic regression analysis. Results There were 90 cases of gallbladder stone in the total population, accounting for 16.2%, including 37 cases of gallbladder stone in IBS group (23.0%) and 53 cases in non-IBS group (13.4%). The prevalence rate of gallbladder stone in IBS group was significantly higher than that in non-IBS group (P<0.05). There were 6 cases of gallbladder muddy stones (3.7%) in IBS group and 3 cases (0.8%) in non-IBS group. And the prevalence rate of gallbladder muddy stones in IBS group was also significantly higher than that in non-IBS group (P<0.05). Logistic regression analysis showed that the age, BMI, total bile acids (TBA), total cholesterol (TC) and combined IBS were independently related to the occurrence of gallbladder stone (P<0.05). In the 161 IBS patients, there were 114 cases of diarrhea-predominant IBS (IBS-D group), including 26 cases (22.8%) of gallbladder stone in IBS-diarrhea (IBS-D, n=114) group and 47 cases of constipation-predominant IBS (IBS-C group), including 11 cases (23.4%) of gallbladder stone. And there were 53 cases (13.4%) of gallbladder stone in the non-IBS group (n=395). Further analysis showed that the prevalence rate of gallbladder stone in IBS-D group was significantly higher than that in non-IBS group (P<0.05). There was no significant difference in gallbladder stone prevalence rate between IBS-C group and non-IBS group (P>0.05). Conclusions There is a correlation between IBS and gallbladder stones. In addition, among the two subtypes of IBS, IBS-D patients may have an increased risk of gallbladder stone compared with non-IBS patients.