PDF
Full
Outline
Objective To observe the effects of Wuzi Yanzong Pill (WYP) on motor function in a mouse model of Parkinson's disease (PD) and to explore its potential mechanisms. Methods Twenty-four male C57BL/6 mice were randomly divided into control group, model group and WYP group, with 8 mice in each group. Mice in model and WYP group were intraperitoneally injected with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine for 7 consecutive days to establish a PD model. From the 1st day of model preparation, mice in WYP group were gavaged with WYP solution [16 g/(kg·d)] twice daily for 14 consecutive days. At the same time, mice in control group and model group were gavaged with 0.9% NaCl solution [50 ml/(kg·d)] twice a day. Gait experiment was utilized to assess the behavioral performance of mice in each group. Immunofluorescence staining was conducted to detect the number of tyrosine hydroxylase (TH)-positive cells in the substantia nigra region, the fluorescence intensity of nuclear factor E2-related factor 2 (Nrf2), and the number of NeuN neurons co-labeled with Nrf2 in each group. Western blotting was employed to determine the expression levels of TH, Kelch-like ECH-associated protein 1 (Keap-1), Nrf2, and heme oxygenase-1 (HO-1) in the brain tissue of mice in each group. Results The gait experiment results showed that, compared with control group, standing time of the left front paw, right front paw, left hind paw, and right hind paw of the mice in model group was significantly shortened (P<0.01), while swinging time of the left front paw, right front paw, left hind paw, and right hind paw was significantly prolonged (P<0.05). Compared with model group, standing time of the left front paw and right hind paw of the mice in WYP group was significantly prolonged (P<0.05), while swing time of the left front paw and right front paw was significantly shortened (P<0.05). Immunofluorescence staining and Western blotting results showed that, compared with control group, in model group the number of TH-positive cells, average fluorescence intensity of Nrf2, and HO-1 levels decreased (P<0.01), while the Keap-1 protein level increased (P<0.01), and the number of Nrf2 expression on NeuN neurons decreased (P<0.001). Compared with model group, the number of TH-positive cells, average fluorescence intensity of Nrf2, HO-1 level, and the number of Nrf2 expression on NeuN neurons in the brain tissue of mice in WYP group increased (P<0.05), while Keap-1 protein level decreased (P<0.05). Conclusions WYP could alleviate the motor dysfunction and protect dopaminergic neurons in PD mice. The underlying mechanism may be related to the regulation of Keap-1/Nrf2/HO-1 pathway to inhibit oxidative stress response.
PDF
109
41
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62199257
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
