PDF
Full
Outline
Objective To investigate the protective effects of paeonol (PAE) on autophagy in human neuroblastoma cells (SH-SY5Y) induced by overexpression of α-synuclein (α-Syn), and to explore its related mechanism. Methods SH-SY5Y cells served as control group, while those induced with A53T-α-Syn mutation were used as model group. Additional groups included PAE (150 μg/ml) group, 3-MA (1 mmol/L) group, and PAE(150 μg/ml)+3-MA (1 mmol/L) group. Cell viability was assessed using CCK-8 method, cell morphology was observed under an optical microscope, and protein expressions of α-Syn, LC3-Ⅱ, p62, Beclin-1, phosphorylated c-Jun N-terminal kinase (p-JNK), and p-Bcl-2 were determined by Western blotting. Results Compared with control group, model control exhibited decreased cell survival (P<0.01), increased α-Syn expression (P<0.001), reduced expression of autophagy-related proteins LC3-Ⅱ and Beclin-1 (P<0.01, P<0.05), elevated autophagy substrate protein p62 (P<0.05), and decreased expression of autophagy pathway-related proteins p-JNK and Bcl-2 (P<0.05, P<0.01). Compared with model group, PAE group showed increased cell survival (P<0.01), decreased α‑Syn and p62 protein expression (P<0.01, P<0.05), and increased expression of LC3-Ⅱ, Beclin-1, p-JNK and Bcl-2 (P<0.05). Compared with PAE group, 3-MA+PAE group demonstrated increased α-Syn expression (P<0.05). Conclusions PAE could attenuate the injury of SH-SY5Y cells induced by A53T-α-Syn and eliminate over-expressed α‑Syn by activating autophagy pathway, which may be associated with the upregulation of JNK/Bcl-2 mediated autophagy pathway.
PDF
158
61
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62199257
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
