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Objective To investigate the protective effect and its mechanism of low-dose interleukin-2 (IL-2) against hepatocyte injury in Concanavalin A (Con A)-induced autoimmune hepatitis (AIH) mice. Methods Eighteen SPF female C57BL/6 mice were randomly divided into normal group, model group and treatment group, each group with 6 mice. Mice in the treatment group were subcutaneously injected with 300 μl 10,000 U IL-2 for 12 d, once a day. 2 h after the last dose, Con A (15 mg/kg) was injected through the tail vein in the model group and treatment group. After 8 h of modeling, the histopathological changes in the mouse liver were observed using HE staining, and the serum levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ) were detected using ELISA method; the expression of apoptotic protein caspase 8/9/3 was detected by Western blotting; and the percentages of Treg and Th1 cells were observed by flow cytometry. Results Compared with normal group, the liver index, spleen index, the percentage of necrotic area of liver tissue, the serum levels of ALT, AST, TNF-α and IFN-γ, and the expression of apoptosis protein caspase 8/9/3 significantly increased in the model group (P<0.05 or P<0.01); Compared with model group, the liver index, spleen index, the percentage of necrotic area of liver tissue, the serum levels of ALT, TNF-α and IFN-γ, and the expression of apoptosis protein caspase 8/9/3 significantly decreased in the treatment group (P<0.05 or P<0.01). The flow cytometry results showed that compared with normal group, the percentages of Treg and Th1 cells and Th1/Treg ratio increased in the model group (P<0.05 or P<0.01); Compared with the model group, the percentage of Treg cells further increased (P<0.01), Th1/Treg ratio decreased significantly in the treatment group (P<0.05), but there was no significant difference in the percentage of Th1 cells between two groups (P>0.05). Conclusion Low-dose of IL-2 can effectively improve liver injury in AIH mice, and the mechanism of action may be related to inducible Treg cell activation.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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