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The effects of high hydrostatic pressure (HHP) treatment at 100-500 MPa for 10-30 min on the solubility and molecular structure characteristics of thermal denatured rice protein dissolved in solvent A (water solution, pH 8.0) and solvent B (containing 6 mol/L urea and 1% mercaptoethanol, pH 8.0) were researched. The results showed that the solubility of rice protein in solvent B was notably improved after HHP treatment, especially at 100 MPa for 20 min, which was from 6.89% at 0.1 MPa up to 18.13%, while in solvent A, it was from 4.37% to 9.60% at the same pressure condition. SDS-PAGE analysis showed that the subunit composition of soluble protein treated under HHP in solvent A had few differences from that of untreated. The similar phenomena could be seen in insoluble protein. However in solvent B, the relative contents of the subunits in soluble changed obviously. The observation of SEM (Scanning Electron Microscope) showed that the surface structure and aggregation state of rice protein could be changed by HHP treatment.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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