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Effects of dynamic high pressure micro-fluidization (DHPM) in the extraction processing on the antioxidative activities of flavonoid from sweet potato leaves by DHPM in contrast to the traditional ethanol extraction were performed to explore the possible mechnism in combination with the high performance liquid chromatography combined with mass spectrum (HPLC-MS). The results showed that pretreatment by DHPM could promote cell rupture and unfolding of cell membrane, which caused the increase in the extraction rate of total flavonoid. The best result was achieved at 100 MPa with the highest yield of 5.440%±0.006%, which was higher by 21.7%, relative to the enthanol extraction. HLPC-MS analysis revealed that pretreatment by DHPM could improve the purity and content of lower polar molecule in extract solvent.80 MPa and 100 MPa pressurization treatment decreased the antioxidant activity of samples with identical flavonoid content, but had negligible effects on elements and structure of flavonoid. Pretreatment with 120 MPa (SF120 sample) pressurization had the strongest antioxidation with IC50 for DPPH and ·OH were (15.99±1.19)mg/L and (0.093±0.012)g/L, respectively. The main flavoniods of SF0, SF80 and SF100 are quercetin, 4′, 7- dimethoxykaempferol, myricetin, ombuin and rhamnocitrin, while, the main flavoniods of SF120 are quercetin, myricetin and ombuin.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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