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Effect of High Pressure Processing on Polyphenol Oxidase from Trametes Trogii
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Xiao-Qiang CHEN1, 2, Yin-Jun ZHANG3, Shi-Kang ZHANG1, Jian-Rong LI2, 6, Jian-Chang JIN4, Na ZHENG5, Xiu-Fang YANG1
Chinese Journal of High Pressure Physics | 2012, 26(2) : 235 - 240
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Chinese Journal of High Pressure Physics | 2012, 26(2): 235-240
Effect of High Pressure Processing on Polyphenol Oxidase from Trametes Trogii
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Xiao-Qiang CHEN1, 2, Yin-Jun ZHANG3, Shi-Kang ZHANG1, Jian-Rong LI2, 6, Jian-Chang JIN4, Na ZHENG5, Xiu-Fang YANG1
Affiliations
  • 1.Hangzhou Tea Research Institute, China COOP, Hangzhou 310016, China
  • 2.College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310035, China
  • 3.College of Biological and Envrionmental Engineering, Zhejiang University of Technology, Hangzhou 310014, China
  • 4.College of Biology and Environmental Engineering, Zhejiang Shuren University, Hangzhou 310015, China
  • 5.State Key Laboratory of Chemical Engineering, Zhejiang University, Hangzhou 310027, China
  • 6.Bohai University, Jinzhou 121013, China
Published: 2012-04-15
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The activity of polyphenol oxidase (PPO) produced from Trametes Trogii was determined through using tea polyphenols as substrate. The pH value and the substrate concentration of strongest activity of PPO are 5.0 and 4 mg/mL, respectively. The activity of PPO processed by ultrahigh pressure of 100, 300 and 500 MPa for 10 min accounted for 93%, 87% and 86% of that of untreated one. The CD spectra of PPO processed by 100 and 300 MPa pressure respectively displayed folding shape and tillering shape of Cotton effect peak located in 190-210 nm, while the retention time of the first peak fraction (i.e., the maximum molecular weight fraction) of the two processed PPO on HPGPC profiles were advanced to 1.514 and 2.296 min, respectively from 6.678 min. Compared with control, CD spectrum and HPGPC of PPO treaded by 500 MPa showed more significant difference with only one positive Cotton effect peaks on CD spectra and reduced peak area of main components on HPGPC profiles (ie, the response value of 280 nm decreased). The disparity of CD and HPGPC of PPO treated by various UHPP showed the corresponding difference in the degree

ultrahigh pressure processing (UHPP)  /  polyphenol oxidase (PPO)  /  circular dichroism (CD)  /  high performance gel permeation chromatography (HPGPC)  /  Trametes Trogii
陈小强, 章银军, 张士康, 励建荣, 金建昌, 郑娜, 杨秀芳. 超高压处理对毛栓菌多酚氧化酶的影响. 高压物理学报, 2012 , 26 (2) : 235 -240 .
Xiao-Qiang CHEN, Yin-Jun ZHANG, Shi-Kang ZHANG, Jian-Rong LI, Jian-Chang JIN, Na ZHENG, Xiu-Fang YANG. Effect of High Pressure Processing on Polyphenol Oxidase from Trametes Trogii[J]. Chinese Journal of High Pressure Physics, 2012 , 26 (2) : 235 -240 .
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Article Info
  • Receive Date：2010-11-17
  • Online Date：2026-11-05
  • Published：2012-04-15
Article Data
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History
  • Received：2010-11-17
  • Revised：2011-02-17
Funding
Affiliations
    1.Hangzhou Tea Research Institute, China COOP, Hangzhou 310016, China
    2.College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310035, China
    3.College of Biological and Envrionmental Engineering, Zhejiang University of Technology, Hangzhou 310014, China
    4.College of Biology and Environmental Engineering, Zhejiang Shuren University, Hangzhou 310015, China
    5.State Key Laboratory of Chemical Engineering, Zhejiang University, Hangzhou 310027, China
    6.Bohai University, Jinzhou 121013, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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