The activity of polyphenol oxidase from red raspberry treated at 600 MPa for 45 min is even up to 40%, indicating strong pressure resistance. In order to further inactivate polyphenol oxidase from red raspberry, the combination effect of high hydrostatic pressure and metal ions on polyphenol oxidase activity was investigated. The change of polyphenol oxidase activity treated at 400 and 600 MPa after adding metal ions was revealed. The activity of raspberry polyphenol oxidase gradually decreases with the pressure increasing and/or the holding time extending with/without adding metal ions. Fe2+, Mn2+ (both inhibited polyphenol oxidase) and Cu2+ (activated polyphenol oxidase) in combination with pressure have synergistic effects, Ca2+ (inhibited polyphenol oxidase) combined with pressure has antagonistic effect, and Zn2+ (not change polyphenol oxidase activity) has no synergistic/antagonistic effect.
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |