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The inactivation kinetics of β-glucosidase in blackberry were investigated at high hydrostatic pressure (300-600 MPa) combined with moderate temperature (35-55 ℃). Analysis of the obtained kinetic data revealed a dual-effect of combined pressure-temperature inactivation: the first one, designated as an instantaneous pressure kill value (IPK), which depended on temperatures and pressures, but was independent of treatment time; and the second one, a labile and stable fraction of β-glucosidase from blackberry are present and the inactivation kinetics of β-glucosidase by high hydrostatic pressure combined with temperatures are adequately described by the two-fraction model. The kinetic rate constants KL and KS of labile and stable fractions are 2.777 and 0.047 min-1, and the decimal reduction times DL and DS are 0.83 and 49.1 min at 400 MPa and 55 ℃. TZ representing the needs of temperature increase for a 90% reduction of the D value and the activation energy Ea of the labile fraction at 400 MPa are 23.36 ℃ and 81.95 kJ/mol, pZ representing the needs of pressure increase for a 90% reduction of the D value and the activation volume Va at 45 ℃ are 1 111 MPa and -5.02 cm3/mol.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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