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Molecular Analysis of Stable Mutagenesis in Cucumber Induced by High Hydrostatic Pressure
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Wei-Guang SHI1, Hui-Jie WU3, Guo-Qiang ZHOU2, Qi-Feng JIANG3, Yin-Qiu CUI2, Xiao-Yang LIU1
Chinese Journal of High Pressure Physics | 2011, 25(4) : 379 - 384
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Chinese Journal of High Pressure Physics | 2011, 25(4): 379-384
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Molecular Analysis of Stable Mutagenesis in Cucumber Induced by High Hydrostatic Pressure
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Wei-Guang SHI1, Hui-Jie WU3, Guo-Qiang ZHOU2, Qi-Feng JIANG3, Yin-Qiu CUI2, Xiao-Yang LIU1
Affiliations
  • 1.State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, College of Chemistry, Jilin University, Changchun 130021, China
  • 2.College of Life Science, Jilin University, Changchun 130021, China
  • 3.Academy of Vegetable and Flower Sciences of Jilin Province, Changchun 130033, China

    • SHI Wei-Guang (1979-), male, doctoral candidates, major in high pressure biology. E-mail:

About Author:

SHI Wei-Guang (1979-), male, doctoral candidates, major in high pressure biology. E-mail:

Published: 2011-08-15
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Two mutant cucumber lines are obtained from the Tianjin cucumber cultivar S9925-3 after treatment with high hydrostatic pressure. DNA fingerprint analyses are performed for the two mutants and their parental wild-type cucumber cultivars by AFLP (Amplified Fragments Length Polymorphism) analysis. A total of 30 randomly selected primer pairs are used for AFLP. Ten specific different bands between the mutants and the wild-type cucumber cultivars are excised from the polyacrylamide gel for DNA sequencing. Alignment of the obtained DNA sequences with NCBI (National Center for Biotechnology Information) database identifies the mutation sites in cucumber genome, which may be the reason of phenotypic changes of the mutant cucumbers. This is the first time that a study has showed at molecular level that treatment with high hydrostatic pressure results in stable mutations in cucumber cultivar. Our results indicate that high hydrostatic pressure-induced mutational breeding can be used as an effective approach for the crop improvement.

high hydrostatic pressure  /  DNA fingerprint  /  cucumber cultivar  /  amplified fragments length polymorphism (AFLP)
施伟光, 吴慧杰, 周国强, 姜奇峰, 崔银秋, 刘晓旸. 高静水压诱导黄瓜稳定遗传变异系的分子分析. 高压物理学报, 2011 , 25 (4) : 379 -384 .
Wei-Guang SHI, Hui-Jie WU, Guo-Qiang ZHOU, Qi-Feng JIANG, Yin-Qiu CUI, Xiao-Yang LIU. Molecular Analysis of Stable Mutagenesis in Cucumber Induced by High Hydrostatic Pressure[J]. Chinese Journal of High Pressure Physics, 2011 , 25 (4) : 379 -384 .
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Funding
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  • National Natural Science Foundation of China(20121103; 20871052)
  • New Century Excellent Talents in University of Ministry of Education of China
References
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[1]
Macgregor R B. The Interactions of Nucleic Acids at Elevated Hydrostatic Pressure[J]. Biochim Biophys Acta, 2002, 1595(1-2): 266-276.
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Kornblatt J A, Kornblatt M J. The Effects of Osmotic and Hydrostatic Pressures on Macromolecular Systems[J]. Biochim Biophys Acta, 2002, 1595(1-2): 30-47.
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Shen S L, Wang Z W, Shan X H, et al. Alterations in DNA Methylation and Genome Structure in Two Rice Mutant Lines Induced by High Pressure[J]. Sci China C Life Sci, 2006, 49(2): 97-104.
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Agarwal M, Shrivastava N, Padh H. Advances in Molecular Marker Techniques and Their Applications in Plant Sciences[J]. Plant Cell Rep, 2008, 27(4): 617-631.
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Doyle J J, Doyle J L. Isolation of Plant DNA from Fresh Tissue[J]. Focus, 1990, 12: 13-15.
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Hossain M B, Awal A, Rahman M A, et al. Distinction between Cold-Sensitive and -Tolerant Jute by DNA Polymorphisms[J]. J Biochem Mol Biol, 2003, 36(5): 427-432.
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Bassam B J, Gresshoff P M. Silver Staining DNA in Polyacrylamide Gels[J]. Nat Protocols, 2007, 2(11): 2649-2654.
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Long L, Lin X, Zhai J, et al. Heritable Alteration in DNA Methylation Pattern Occurred Specifically at Mobile Elements in Rice Plants Following Hydrostatic Pressurization[J]. Biochem Biophys Res Commun, 2006, 340(2): 369-376.
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Mañas P, Mackey B M. Morphological and Physiological Changes Induced by High Hydrostatic Pressure in Exponential- and Stationary-Phase Cells of Escherichia coli: Relationship with Cell Death[J]. Appl Environ Microbiol, 2004, 70(3): 1545-1554.
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Arroyo G, Sanz P D, Préstamo G. Response to High-Pressure, Low-Temperature Treatment in Vegetables: Determination of Survival Rates of Microbial Populations Using Flow Cytometry and Detection of Peroxidase Activity Using Confocal Microscopy[J]. J Appl Microbiol, 1999, 86(3): 544-556.
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Appendix
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Year 2011 volume 25 Issue 4
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Article Info
  • Receive Date：2010-03-16
  • Online Date：2026-11-02
  • Published：2011-08-15
Article Data
Affiliations
History
  • Received：2010-03-16
  • Revised：2010-09-28
Funding
National Natural Science Foundation of China(20121103; 20871052)
New Century Excellent Talents in University of Ministry of Education of China
Affiliations
    1.State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, College of Chemistry, Jilin University, Changchun 130021, China
    2.College of Life Science, Jilin University, Changchun 130021, China
    3.Academy of Vegetable and Flower Sciences of Jilin Province, Changchun 130033, China

Corresponding:

LIU Xiao-Yang (1964-), male, Ph.D., professor, Ph.D. supervisor, major in high pressure chemistry and physics. E-mail:
CUI Yin-Qiu (1972-), female, Ph.D., associate professor, major in biochemistry and molecular biology. E-mail:
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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