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We studied the effect of high pressure process (HPP) on exoproteinase of Bacillus subtilis by using Box-Behnken response surface methodology. Experimental results showed that HPP could inhibit activity of exoproteinase significantly. In the course of treatment, pressure and temperature were key factors to passivate enzyme, and their interaction was notable. A model of exoproteinase passivation was established by design expert software, which determination coefficient was 0.974 7 and adjusted determination coefficient was 0.942 1. Analysis of variance implied that the model was significant. Therefore, the model could be used to analyze and predict. Additionally, the experiment indicated that exoproteinase activity correlated with lethal rate significantly (R=0.933 0). The exoproteinase could be regarded as an index to evaluate effect of HPP on microorganism.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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