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Three groups of short annual Catharanthus roseus seeds were treated in high pressure nitrogen gas for 4 h at room temperature; the pressure was 10, 20 and 30 MPa, respectively. Other untreated seeds were regarded as a control group (abbreviated to CP). All seeds were planted under same condition. The vegetal character of the Catharanthus roseus was observed in detail and contents of Vinblastine and Vincristine in the plants were detected by HPLC. Experimental results indicated that the contents of Vinblastine increased remarkably and Vincristine tended to increase in the plants of the pressure treated groups in comparison to CP. It is also noticed that distributions of variance of the flowers and ill aftergrowth plants are corresponding approximately to that of the contents of Vinblastine. Mechanism of the effects of pressure treatment was discussed qualitatively.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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