After up to 10 generations of purification by strict self-fertilizing, seeds of two rice varieties (Oryza sativa L.), Yuexiangzhan and Bijing-38 were treated with high hydrostatic pressure during last 5 years, and 5 contemporaneous mutants and 4 descendant natural mutants were obtained with high reproducibility for the contemporaneous mutants. To verify if the mutation was take place at DNA level, Inter Simple Sequence Repeat (ISSR) analysis of the original rice varieties and mutants was conducted. The analysis revealed that the genetic substance (DNA) had been altered. Evidently, the mutation was induced by high hydrostatic pressure treatment. Generally, the mutation took place contemporaneously and the new characters are of high genetic stability; the further the genetic distance between the mutant and the original is, the higher the genetic stability of the mutant is.
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |