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Effect of High Hydrostatic Pressure on Seeds Germination and Seedling Isoenzyme in Rice (Oryza sativa L.)
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Cheng-Ke BAI1, Gui-Shuang LI1, Jun DUAN1, Chang-Lian PENG1, Zhong-Gang DUAN1, Ke-Nan WENG2, Shi-Ping XU2
Chinese Journal of High Pressure Physics | 2003, 17(4) : 283 - 289
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Chinese Journal of High Pressure Physics | 2003, 17(4): 283-289
Effect of High Hydrostatic Pressure on Seeds Germination and Seedling Isoenzyme in Rice (Oryza sativa L.)
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Cheng-Ke BAI1, Gui-Shuang LI1, Jun DUAN1, Chang-Lian PENG1, Zhong-Gang DUAN1, Ke-Nan WENG2, Shi-Ping XU2
Affiliations
  • 1.South China Institute of Botany, Chinese Academy of Sciences, Guangzhou 510650, China
  • 2.Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China
Published: 2003-12-05
Outline
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Three indica rice cultivars seeds, Yuexiangzhan were treated by different high hydrostatic pressure or were kept for different time. As comparison to control, both germination rate and seedling rate decreased considerably with pressure or treated time increasing. Plant growth was inhibited in treated materials during the early growth stage of seedling, but the plant height of treated cultivars could exceed that of control after sowing 30 d. The expression of some isoenzymes, esterase (EST), peroxidase (POX), superoxide dismutase (SOD) and malic enzyme (ME), was studied by polyacrylamide gel electrophoresis (PAGE), the results indicated that the expression of EST was restrained, whereas the other isoenzymes was affected lettle on expression of POX, SOD and ME. Three types of rice (Oryza sativa L.) cultivars seeds, indica cultivars (Nanjing 11 and Yuexiangzhan), japonica cultivars (Qiuguang and Taizhong 65) and intermediate type between indica cultivars and japonoca cultivars (Bulu Gendjah and Bulu Rusa), were treated by 75 MPa pressure for 10 h. The germination rate and seedling rate of three types rice all decreased. But the decrease was most significant in two indica cultivars (Nanjing 11 and Yuexiangzhan), whereas was least in two japonica cultivars (Qiuguang and Taizhong 65). The results implied that different types of rice cultivars exhibited different responses to high hydrostatic pressure. Japinica cultivars were higher resistance to high pressure than other types of cultivars. The above results showed that high hydrostatic pressure could affect the seeds germination, seedling growth and expression of EST.

high hydrostatic pressure  /  rice seeds  /  isoenzyme
白成科, 李桂双, 段俊, 彭长连, 段中岗, 翁克难, 徐世平. 高静水压对水稻种子萌发及同工酶的影响. 高压物理学报, 2003 , 17 (4) : 283 -289 .
Cheng-Ke BAI, Gui-Shuang LI, Jun DUAN, Chang-Lian PENG, Zhong-Gang DUAN, Ke-Nan WENG, Shi-Ping XU. Effect of High Hydrostatic Pressure on Seeds Germination and Seedling Isoenzyme in Rice (Oryza sativa L.)[J]. Chinese Journal of High Pressure Physics, 2003 , 17 (4) : 283 -289 .
Year 2003 volume 17 Issue 4
25
5
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  • Receive Date:2003-03-05
  • Online Date:2026-10-31
  • Published:2003-12-05
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  • Received:2003-03-05
  • Revised:2003-06-09
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Affiliations
    1.South China Institute of Botany, Chinese Academy of Sciences, Guangzhou 510650, China
    2.Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China
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表12种不同金属材料的力学参数
科
Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)
属
Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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