Three indica rice cultivars seeds, Yuexiangzhan were treated by different high hydrostatic pressure or were kept for different time. As comparison to control, both germination rate and seedling rate decreased considerably with pressure or treated time increasing. Plant growth was inhibited in treated materials during the early growth stage of seedling, but the plant height of treated cultivars could exceed that of control after sowing 30 d. The expression of some isoenzymes, esterase (EST), peroxidase (POX), superoxide dismutase (SOD) and malic enzyme (ME), was studied by polyacrylamide gel electrophoresis (PAGE), the results indicated that the expression of EST was restrained, whereas the other isoenzymes was affected lettle on expression of POX, SOD and ME. Three types of rice (Oryza sativa L.) cultivars seeds, indica cultivars (Nanjing 11 and Yuexiangzhan), japonica cultivars (Qiuguang and Taizhong 65) and intermediate type between indica cultivars and japonoca cultivars (Bulu Gendjah and Bulu Rusa), were treated by 75 MPa pressure for 10 h. The germination rate and seedling rate of three types rice all decreased. But the decrease was most significant in two indica cultivars (Nanjing 11 and Yuexiangzhan), whereas was least in two japonica cultivars (Qiuguang and Taizhong 65). The results implied that different types of rice cultivars exhibited different responses to high hydrostatic pressure. Japinica cultivars were higher resistance to high pressure than other types of cultivars. The above results showed that high hydrostatic pressure could affect the seeds germination, seedling growth and expression of EST.
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |