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Outline
In order to investigate the effects of ultra high pressure (UHP) on the content of soluble protein, peroxidase (POD) and pectinmethylesterase (PME) in litchi fruit, the pulp of litchi (Litchi chinensis Sonn cv. Huaizhi) was subjected to UHP treatment (100~400 MPa for 30 min at 10 ℃). The activities of POD and PME were detected by spectrophotometry, the isoenzymes of POD and PME were detected by native-PAGE and active staining. The content of soluble protein was detected by SDS-PAGE. Results showed that the POD activity raised and the new isoenzyme appeared when a treatment of 100~200 MPa was applied, the treatment of pressure 300~400 MPa made the POD activity decrease and the new isoenzyme disappear. The PME activity ascended and the new isoenzyme of PME appeared at 100 MPa; when the treatment of 200~400 MPa was applied the PME activity lowered and the new isoenzyme disappeared. The content of soluble protein had a little increase at 100 MPa, but continue to declined at 200~400 MPa.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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